Abstract: Objective：To study the surface characteristics and biocompatibility of four kinds of biological membranes in order to compare their applications of cell adhesion and proliferation，as well as interaction between materials and membrane types. Methods：By casting or electrospinning, we fabricated poly(ε-caprolactone)(PCL) membranes and poly(lactic-co-glycolic acid)(PLGA) membranes. Field emission scanning electron microscope (FESEM) was used to detect the surface microstructure of the membranes. After Cell line MC3T3-E1 was seeding to all membranes, we tested cell proliferation of 1,3,7days by Cell counting Kit-8 (CCK-8) assay, while estimating cell adhesion by confocal laser scanning microscope (CLSM) and real-time quantitative polymerase chain reaction (RT-qPCR). Results：FESEM pictures showed pores on cast membranes, while interwoven fibers were observed on electrospun membrane surfaces. CCK-8 tests showed MC3T3-E1cells prefer two PLGA groups than PCL groups（P<0.05）, no matter how the membranes were fabricated（P＞0.05）. Despite PCL electrospun membranes, cells were observed spreading well on other membranes by CLSM. RT-qPCR tests showed there are interactions between materials and membrane types, and PCL cast membrane group showed highest integrin gene expression（P<0.05）. Conclusions：PLGA membranes contributed to MC3T3-E1 cell proliferation, while PCL cast membranes were suitable for cellular adhesion. Other than singly using one kind of material or fabrication method, combination use of PLGA, PCL by casting or electrospinning will become the trend to fabricate biological membranes.