›› 2015, Vol. 6 ›› Issue (2): 57-61.

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Expression of BMP7 during the developing deciduous molar tooth of miniature pig

  

  • Received:2015-03-27 Revised:2015-05-12 Online:2015-06-25 Published:2015-07-13

Abstract: Objective:To study the expression patterns of bone morphogenetic proteins 7 (BMP7) during deciduous molar tooth development in miniature pig. Methods: The staged miniature pig embryos at embryonic day (E) 40th day, 50th day, 60th day were collected. Mandibles were fixed, decalcified and embedded before section. HE staining, immunohistochemistry, in situ hybridization using specific radionuclide labeled cRNA probes and real-time PCR were used to detect BMPs expression patterns during morphogenesis of the third deciduous molar tooth of miniature pig. Results:HE staining showed that the deciduous third molar at (E) 40th day, 50th day, 60th day represent the cap stage, bell stage and late bell stage (or secretory phase), respectively. Immunohistochemical staining showed BMP7 were expressed in both enamel organ and dental mesenchyme at E40 day and E50 day. However, at E60 day, expression of BMP7 was mainly located in pre-odontoblasts and pre-ameloblasts. Conclusions:BMP7 were temporal and spatial expressed during swine deciduous molar development, suggesting these molecules are associated with tooth morphogenesis and cell differentiation.