Abstract: Objective：To characterize and observe the effect of Osterix on the osteogenic differentiation of mouse iPSCs. Methods： Through iPS cell culture, gene transfection, semi-quantitative RT-PCR and histology, we analyzed the alteration of osteogenesis related genes and protein in the mouse iPSCs before and after Osterix transfection. Results：Suspension of iPS cells in differentiation culture (drop) can form embryoid bodies (EB). After the plasmid transfection, the potential of multi-directional differentiation of iPS cells was not found to have changed. Moreover, compared with the control group, the activity of ALP in the formation of mineralized nodules, osteogenesis related genes (Runx2, bone sialoprotein and osteocalcin) expression were significantly enhanced in the iPSCs transfected with Osterix (P<0.05). Conclusions：iPSCs can be differentiated to bone cells, and the transcription factor Osterix can promote the osteogenic differentiation of iPS cells.