Abstract: Objective：To research the effect of xeno-ADM (xeno-acellular dermal matrix) on vasculogenesis of human umbilical vein endothelial cell(HUVEC).Methods：Porcine acellular dermal matrix (P-ADM) and bovine acellular dermal matrix (B-ADM) were co-cultured with HUVEC, using blank and Medical collagen membrane(Bio-Gide) as control. The concentration of vascular endothelial growth factor (VEGF) collected from different groups at 4, 6, 12, 24, 48, 96 h was determined by ELISA test. Meanwhile, the number of cellular casts were counted by observing HUVEC infected by GFP lentivirus. Results：The VEGF concentration of xeno-ADM groups were higher than that of the control groups（P<0.05）after 24 h, and P-ADM was more than that of the control groups after 48 h（P<0.05）.The number of cellular casts of P-ADM group was about as twice as that of the control group after 24 h. The B-ADM group improved less than the P-ADM group. No cellular cast was found in the control group..Conclusions：Xeno-ADM has better effect on vasculogenesis than traditional medical collagen membrane, which suggesting that it has the potential to be the next generation of surgical material applying to mucogingival therapy.