Abstract: Objective To explore the effect of transcription factor GATA4 on bone formation and mineralization. Methods Wnt1-Cre mice were mated to GATA4fl/fl mice by cre-loxp gene conditional knockout technique, and conditional knockout GATA4 gene of neural crest cell mice (Wnt1-Cre;GATA4fl/fl) were produced. 2-week-old GATA4 conditional knockout (CKO) mice and wild type (WT) mice were sacrificed and then Micro-CT was conducted to compare the size and volume of craniofacial bones such as calvarial bone, palate bone and right mandible bone. Results Compared with WT mice, the skull bone, palate bone and mandible bone of CKO mice were smaller, and the width of midpalatal suture was increased. The mandible of CKO mice also showed decreased BV/TV and Tb.N (P<0.05) values, and increased Tb.Sp value; Tb.N was not significantly different (P>0. 05). Conclusions These results indicate an important role of GATA4 in craniofacial bone development and mineralization.