›› 2017, Vol. 8 ›› Issue (1): 25-27.

Previous Articles     Next Articles

Effect of Porphyromonas gingivalis lipopolysaccharide on the of EphA4 gene in ST-2 Cells

  

  1. 1. Department of Dental Implantology, School and Hospital of Stomatology, Jilin University
    2. Stomatological Hospital, Jilin University
  • Received:2016-12-13 Revised:2017-02-24 Online:2017-03-25 Published:2017-04-11

Abstract: Objective:To observe the effect of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the of EphA4 in mouse marrow stromal cell line ST-2. Methods:ST-2 cells were co-cultured with 10 μg/mL of Pg-LPS at 1 d, 3 d and 7 d. EphA4 gene and the osteogenic related genes (Runx2, ColⅠ and ALP) were detected by RT-PCR. Results:Compared with Pg-LPS group, at the 1 d, 3 d and 7 d, the EphA4 mRNA was significantly decreased by 2.5 folds,2.4 folds and 2.3 folds in control group. At the same time,the osteogenic related gene Runx2 in the experimental group were significantly decreased (P < 0.01) as compared with that of the control group at 1 d and 3 d, but on the 7 d this was not obvious; as to the osteogenic related genes ColⅠand ALP s in 1 d, 3 d and 7 d, the experimental group was less than the control group, and the difference was statistically significant (P<0.01). Conclusions:Pg-LPS can inhibit the s of EphA4 gene and osteogenic related genes Runx2, ColⅠand ALP in ST-2 cells in the osteogenetic differentiation.

CLC Number: