Abstract: Objective：To explore the proliferation and odonto/osteogenic potential of DPSCs separated from GK rat with hyperglycemia. Methods： The 3-month-old GK rat (one diabetic model rat) and Wistar rat were selected. The DPSCs were isolated and cultured when the blood glucose level had been stable for 1 weeks. MTT assay was applied to estimate the proliferation of the two DPSCs. The odonto/osteogenic markers (OCN, OSX and DMP1) of the two DPSCs were measured by real-time RT-PCR and Western blot. Results： The level of blood glucose in GK rats remained about 20.1~22.3 mmol/L (high glucose) and the level of blood glucose of Wistar rats was 5.8 ~ 6.3 mmol/L (normal glucose level). MTT assay showed the proliferative activity of DPSCs from GK was significantly lower than that of the Wistar rat. Moreover, the expressions of OCN, OSX and DMP1 in DPSCs of GK rats were significantly weaker than those in Wistar rats. Conclusions：The DPSCs from the donor with hyperglycemia or diabetes exhibited the weaker proliferation and odonto/osteogenic potential.