›› 2020, Vol. 11 ›› Issue (1): 9-12.

Previous Articles     Next Articles

IGF-1 enhance the cementoblastic differentiation of Hertwig’s epithelial root sheath cells of rats

  

  • Received:2019-11-04 Revised:2020-02-24 Online:2020-03-25 Published:2020-04-07
  • Contact: Wei-Dong TIAN E-mail:drtwd@sina.com

Abstract: Objective:To study the effects of IGF-1 on the proliferation and differentiation of Hertwig’s epithelial root sheath(HERS) in vitro.Methods:HERS cells were isolated and cultured in vitro, and were treated with IGF-1. Proliferation of HERS cells was evaluated by CCK8 assay. Immunofluorescence staining detected the expression of CK14, E-cadherin, Vimentin of HERS cells. Real-time PCR detected the expression of E-cadherin, N-cadherin, Vimentin, Bsp,Col1, Runx2, Alp. And osteogenic differentiation of HERS cells was determined by Alizarin red staining.Results:HERS cells could have the expression of the marker gene of epithelia and mesenchyme.IGF-1 treatment significantly decreased the expression of epithelial marker gene and increased the expression of mesenchymal marker gene and enhanced the cell proliferation. HERS cells culturedin mineralization-induction medium showed significantly upregulated the expression of genes related to the cementoblastic differentiation such as Bsp,Col1, Runx2, Alp on day 7 and day 14(P<0.05). IGF-1 treatment also significantly enhanced the mineralization nodule formation of HERS cells. Conclusions:IGF-1 regulated the proliferation, cementoblastic differentiation and EMT of HERS cells.

Key words: Hertwig’s epithelial root sheath, IGF-1, Cementoblast