Abstract: Objective:?To investigate the effect of chloromethyl-benzamidodialkylcarbocyanine(CM-Dil) on the proliferation and differentiation of human gingival mesenchymal stem cells(hGMSCs) in vitro and the duration of fluorescence of CM-Dil in vivo. Methods:?hGMSCs were primary cultured by tissue mass method, and surface markers were detected by flow cytometry. After hGMSCs were labeled by CM-Dil, the proliferation was analyzed using CCK-8 assay. Osteogenic differentiation of hGMSCs was investigated using Alizarin Red staining, lipid-forming ability of hGMSCs was detected by Oil Red O staining. The effect of CM-Dil-labeled hGMSCs in vivo was detected in rat skull defect experiment. Results:?The hGMSCs were positive for CD105, CD90 and CD73, but negative for CD14, CD34, CD19, CD45 and HLA-DR. Comparing with the control group, CM-Dil did not affect the proliferation of hGMSCs (P＞0.05), nor did it affect the osteogenic and lipogenic differentiation ability of hGMSCs. In vivo the CM-Dil-labeled hGMSCs could still be observed at week 8. Conclusions:?CM-Dil does not affect the characteristics of hGMSCs in vitro, and the CM-Dil-labeled hGMSCs can be observed for up to 8 weeks in vivo, and CM-Dil can be used as a tracking label of MSC in vivo in the future.

Key words: CM-Dil, gingival mesenchymal stem cells, cell lableing, skull defect model