Abstract: Objective: The aim of this study was to explore the short-term effects of non-surgical periodontal treatment (NSPT) on NO metabolism and blood pressure control. Methods: Forty-five normotensive patients with periodontitis (stage Ⅲ or Ⅳ and grade B or C) were recruited and received NSPT. Periodontal parameters, including plaque index, gingival index, probing depth, clinical attachment loss (CAL) and blood pressure were recorded before and one month after NSPT. Levels of NO in salivary and plasma were explored by Griess reaction. Quantities of Porphyromonas gingivalis (P. gingivalis), Prevotella intermedia (P. intermedia) and total bacteria, in subgingival plaque were determined by real-time PCR. Moreover, levels of C-reactive protein (CRP) in serum and IL-6 in gingival crevicular fluid (GCF) and serum were detected by ELISA. The correlation of blood pressure, periodontal parameters, quantities of bacteria and levels of NaNO2, CRP and IL-6 was analyzed. Results:One month after NSPT, periodontal parameters and blood pressure were decreased (P＜0.05), and there were lower levels of NO in both saliva and plasma (P<0.05). In addition, NSPT decreased the amounts of total bacteria, P. gingivalis, P. intermedia, and the production of IL-6 and CRP (P<0.05). Moderate correlations were revealed between systolic blood pressure and IL-6 level in GCF, salivary NaNO2 level and CAL and there was a moderate correlation between diastolic blood pressure and total bacteria level, P. intermedia (P＜0.05). Conclusions:NSPT decreased NO levels and suppressed systemic and periodontal inflammations, which provided reference for the treatment of periodontitis patients with hypertension.