Abstract: Objective:?To explore the role of human adipose-derived stem cells (hADSCs) in skin wound healing and to investigate the potential mechanism. Methods:?Nude mice skin trauma models were constructed and subsequently divided into hADSCs group, and control group. The trauma size was observed and recorded at the indicated time. HE staining was performed to observe the regeneration of the epithelium. The real time quantitative PCR and Western blot were also used for the detection of Notch downstream pathway expression level. The hADSCs and HaCaT cells were co-cultured and were transfected with Jagged1 and Notch1 overexpression plasmid respectively, and Notch downstream pathway expression levels were detected by real time quantitative RT-PCR and Western blot. The proliferation and migration of HaCaT cells were detected by CCK8, transwell and scratch test. Results: All groups began to scab after 3 days of the molding. The healing rate was significantly higher than control group on day 21, respectively (P<0.05). The regenerated epidermis of hADSCs group was significantly higher than control group on day 4 and day 7 after the molding (P<0.05). And Notch downstream pathway expression level was significantly higher in the hADSCs group (P<0.05). After transfection with overexpression plasmid and co-culture, the proliferation and migration ability of HaCaT cells were significantly enhanced, Notch pathways were activated. Conclusions:?hADSCs can promote skin wound healing process through Jagged1/Notch signaling pathwa