Abstract: Objective:To investigate the effect of self-oxygenated antibacterial photodynamic nanoplatform--F@Ce6-M on inflammation regulation and osteogenic differentiation. Methods:The mouse fibroblast L929 was treated with Porphyromonas gingivalis (P. gingivalis) bacterial solution by light irradiation, and the inflammation model was established. F@Ce6 and F@Ce6-M were respectively added, and the corresponding control group was set up. The gene expression levels of pro-inflammatory and anti-inflammatory factors were detected by qRT-PCR to evaluate the effect of F@Ce6-M mediated antimicrobial photodynamic therapy (aPDT) on inflammation regulation. NF-κB/p65 subunit translocation was detected by immunofluorescence to analyze the immunomodulatory mechanism of F@Ce6-M. The expression of osteoblast-related genes was detected by qRT-PCR and stained with ALP to evaluate the regulatory effect of F@Ce6-M on osteogenic differentiation of preosteoblasts. Results:qRT-PCR results showed that F@Ce6-M mediated aPDT significantly decreased the expression of interleukin (IL-6), IL-1β, tumor necrosis factor (TNF)-α gene compared with negative control group, and the effect was significantly better than that of F@Ce6-M no light group (P<0.05); There were no significant differences in the gene expressions of transforming growth factor (TGF)-β1, arginine (Arg)-1 and IL-10 among all groups (P>0.05). F@Ce6-M light group inhibited NF-κB/p65 subunit translocation (P<0.05). The osteogenic marker expressions of ALP, runt-related transcription factor 2 (RUNX2), collagen type Ⅰ (COL-1) and osteocalcin (OCN) in light group were significantly increased compared with negative control group, and the gene expression levels in F@Ce6-M group were the most significantly increased (P<0.05). The activity level of ALP in the light group was significantly increased, the most significantly increased in the F@Ce6-M light group (P<0.05). Conclusions:F@Ce6-M could reduce the release of pro-inflammatory factors, and significantly restore the osteogenic potential of osteoblasts, thereby enhancing the effect of aPDT on periodontitis.

Key words: antibacterial photodynamic therapy, Periodontitis, inflammatory factors, immunomodulation, osteogenesis differentiation