PLA/PVP-GEL复合支架对MC3T3-E1细胞生物学行为的影响及成骨性能的初探

Abstract

Abstract: Objective:To clarify the effects of polylactic acid (PLA) composite gelatin (GEL) modified with different ratios of polyvinylpyrrolidone (PVP) composite on the biological behavior and osteogenic properties of osteoblasts MC3T3-E1, and to lay the research foundation for the study of scaffolds in the direction of drug delivery for bone tissue engineering. Methods:The PLA/PVP-GEL composite scaffold was prepared by vacuum freeze-drying method, in which the mass ratio of PLA/PVP was determined to be 10:0, 8:2, 7:3, 5:5, and the composite volume ratio of GEL to PLA/PVP was fixed at 5%. The physicochemical properties of the composites were characterized using scanning electron microscopy, infrared spectroscopy and contact angle measurement, and the swelling rate, water absorption and porosity of the composite scaffolds were determined. The biocompatibility of each group of scaffolds was compared using CCK-8 method, and the adhesion pattern and distribution of MC3T3-E1 on each group of scaffolds were observed by immunofluorescence staining. The effects of each group of scaffolds on osteogenic differentiation of MC3T3-E1 cells were investigated by qualitative and quantitative analysis of alkaline phosphatase (ALP) and alizarin red staining. Results:The composite scaffolds with irregular porous morphology could be prepared by vacuum freeze-drying method, and the hydrophilicity of scaffolds with different ratios of PLA/GEL composite PVP was improved (P<0.05). MC3T3-E1 could adhere to each composite scaffold with different ratios, and the 8:2 PLA/PVP-GEL composite scaffold had the best cell adhesion morphology and proliferation (P<0.05).The qualitative staining results of ALP and alizarin red were darker in all groups of PLA/PVP-GEL scaffolds compared to the PLA/GEL scaffold group, all groups of scaffolds had positive effects on the osteogenic differentiation of MC3T3-E1, and the 8:2 PLA/PVP-GEL composite scaffold had the best promotion of extracellular mineralization level of MC3T3-E1 (P<0.05). Conclusions:The 8:2 PLA/PVP-GEL composite scaffold can be used as a basic drug-loaded scaffold for bone tissue engineering and subsequent drug loading studies.

Key words: Poly(lactic acid)/poly(vinyl pyrrolidone), Gelatin, Composite scaffold, Osteogenic differentiation

References 25

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Effect of PLA/PVP-GEL composite scaffold on the biological behavior and osteogenic properties of MC3T3-E1 cell

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