Oral Biomedicine ›› 2025, Vol. 16 ›› Issue (1): 20-25.

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Expression and function of METTL5 in oral squamous cell carcinoma

Yu XingFeng1, Shuang-Yue ZHANG   

  • Received:2024-11-15 Revised:2024-12-03 Online:2025-02-25 Published:2025-03-07
  • Contact: Shuang-Yue ZHANG E-mail:hayyzhshy@njmu.edu.cn

Abstract: Objective:?To determine the expression pattern, clinical significance and biological function of METTL5 in oral squamous cell carcinoma (OSCC). Methods:?Bioinformatic interrogations and immunohistochemical (IHC) staining were utilized for determining the expression and localization of METTL5 in OSCC, and for revealing the relationship between METTL5 expression and prognosis in OSCC patients. The association between the abundance of METTL5 protein and clinicopathological parameters of OSCC patients were evaluated by Chi-square test. CCK-8, colony formation, wound healing and transwell assays were utilized to assess the changes in cellular malignant phenotype after METTL5 silencing in Cal27 cells. Results: METTL5 was upregulated in OSCC, and its aberrant overexpression was positively correlated with high pathological grade, cervical lymph node metastasis and reduced survival in OSCC patients (P=0.0337, 0.0138, P<0.001). METTL5 knockdown significantly inhibited proliferation, migration and invasion of Cal27 cells. Conclusions:?Our findings reveal that METTL5 promotes OSCC progression and acts as a biomarker to predict prognosis of OSCC patients.

Key words: Oral squamous cell carcinoma, METTL5, Prognosis