酪蛋白裂解酶P对口腔致病菌内环境稳态及毒力影响的研究进展

Abstract

Abstract: Caseinolytic protease (Clp) P is widely present in various bacteria, responsible for maintaining the protein homeostasis in bacteria, degrading the misfolded or abnormally accumulated proteins recognized by the regulatory subunit adenosine triphosphate (ATP) dependent endopeptidase Clp. By regulating the expression of related genes and protein substrates, ClpP maintains the bacteria homeostasis, participates in the formation of cell walls and biofilms, as well as in the resistance of complex stress, thus affects the biological function of bacteria. This review elaborates the effects of ClpP protease on the biological behavior and homeostasis regulatory mechanism of various oral pathogenic bacteria.

辛同王 Yi Liu. Advances in the effect of ClpP protease on homeostasis and virulence in oral pathogens[J]. Oral Biomedicine, 2025, 16(1): 53-56.

References 43

[1]	Ripstein ZA, Vahidi S, Houry WA, et al. A processive rotary mechanism couples substrate unfolding and proteolysis in the ClpXP degradation machinery [J]. Elife, 2020, 9:e52158.
[2]	Zheng J, Wu Y, Lin Z, et al. ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of Enterococcus faecalis[J]. BMC Microbiol, 2020, 20(1):30.
[3]	He L, Wang H, Zhang R, et al. The regulation of Porphyromonas gingivalis biofilm formation by ClpP[J]. Biochem Biophys Res Commun, 2019,509(2):335-340.
[4]	Mawla GD, Hall BM, Cárcamo-Oyarce G, et al. ClpP1P2 peptidase activity promotes biofilm formation in Pseudomonas aeruginosa[J]. Mol Microbiol, 2021,115(6):1094-1109.
[5]	Culp EJ, Sychantha D, Hobson C, et al. ClpP inhibitors are produced by a widespread family of bacterial gene clusters[J]. Nat Microbiol, 2022,7(3):451-462.
[6]	陈林,蓝林华,何海栋,等. ClpP蛋白酶研究进展:从细菌到人线粒体[J].中国细胞生物学学报,2014,36(6):717-725.
[7]	Illigmann A, Thoma Y, Pan S, et al. Contribution of the Clp Protease to Bacterial Survival and Mitochondrial Homoeostasis[J]. Microb Physiol, 2021,31(3):260-279.
[8]	Aljghami ME, Barghash MM, Majaesic E, et al. Cellular functions of the ClpP protease impacting bacterial virulence[J]. Front Mol Biosci, 2022,9:1054408.
[9]	Dayananda A, Dennison TSH, Fonseka HYY, et al. Allosteric communication in the gating mechanism for controlled protein degradation by the bacterial ClpP peptidase[J]. J Chem Phys, 2023,158(12):125101.
[10]	Kong X, Wang B, Chen X, et al. Hinokiflavone Attenuates the Virulence of Methicillin-Resistant Staphylococcus aureus by Targeting Caseinolytic Protease P[J]. Antimicrob Agents Chemother, 2022,66(8):e0024022.
[11]	Hou XH, Zhang JQ, Song XY, et al. Contribution of ClpP to stress tolerance and virulence properties of Streptococcus mutans[J]. J Basic Microbiol, 2014,54(11):1222-1232.
[12]	Feng Y, Wang H, Lu HE, et al. Effects of ClpP protease on biofilm formation of Enterococcus faecalis[J]. J Appl Oral Sci, 2021,29:e20200733.
[13]	Wang C, Li M, Dong D, et al. Role of ClpP in biofilm formation and virulence of Staphylococcus epidermidis[J]. Microbes Infect, 2007, 9(11): p. 1376-1383.
[14]	Zhang JQ, Hou XH, Song XY, et al. ClpP affects biofilm formation of Streptococcus mutans differently in the presence of cariogenic carbohydrates through regulating gtfBC and ftf[J]. Curr Microbiol, 2015,70(5):716-723.
[15]	Liu Q, Wang X, Qin J, et al. The ATP-Dependent Protease ClpP Inhibits Biofilm Formation by Regulating Agr and Cell Wall Hydrolase Sle1 in Staphylococcus aureus[J]. Front Cell Infect Microbiol, 2017,7:181.
[16]	Lemos JA, Burne RA. Regulation and Physiological Significance of ClpC and ClpP in Streptococcus mutans[J]. J Bacteriol, 2002,184(22):6357-6366.
[17]	Roy S, Zhu Y, Ma J, et al. Role of ClpX and ClpP in Streptococcus suis serotype 2 stress tolerance and virulence[J]. Microbiol Res, 2019,223-225:99-109.
[18]	Fei X, Bell TA, Barkow SR, et al. Structural basis of ClpXP recognition and unfolding of ssrA-tagged substrates[J]. Elife, 2020,9: e61496.
[19]	Tian XL, Li M, Scinocca Z, et al. ClpP is required for proteolytic regulation of type II toxin-antitoxin systems and persister cell formation in Streptococcus mutans[J]. Access Microbiol, 2019,1(8):e000054.
[20]	Xu W, Gao W, Bu Q, et al. Degradation Mechanism of AAA+ Proteases and Regulation of Streptomyces Metabolism[J]. Biomolecules, 2022,12(12):1848.
[21]	杨瑞瑞,吴旭日,宋潇达. ssrA标签及其变体在细菌蛋白质降解中的研究与应用进展[J]. 药物生物技术,2023,30(4):434-440.
[22]	Bhardwaj S, Roy KK. ClpP Peptidase as a Plausible Target for the Discovery of Novel Antibiotics[J]. Curr Drug Targets, 2024,25(2):108-120.
[23]	Ghanbarpour A, Cohen SE, Fei X, et al. A closed translocation channel in the substrate-free AAA+ ClpXP protease diminishes rogue degradation[J]. Nat Commun, 2023,14(1):7281.
[24]	Mabanglo MF, Houry WA. Recent structural insights into the mechanism of ClpP protease regulation by AAA+ chaperones and small molecules[J]. J Biol Chem, 2022,298(5):101781.
[25]	Gurung V, Biswas I. ClpX/P-Dependent Degradation of Novel Substrates in Streptococcus mutans[J]. J Bacteriol, 2022,204(4):e0059421.
[26]	Ortiz C, Natale P, Cueto L, et al. The keepers of the ring: regulators of FtsZ assembly[J]. FEMS Microbiol Rev, 2016,40(1):57-67.
[27]	Pazos M, Natale P, Vicente M. A specific role for the ZipA protein in cell division: stabilization of the FtsZ protein[J]. J Biol Chem, 2013,288(5):3219-3226.
[28]	Capela R, Félix R, Clariano M, et al. Target Identification in Anti-Tuberculosis Drug Discovery[J]. Int J Mol Sci, 2023,24(13): 10482.
[29]	Kock H, Gerth U, Hecker M. MurAA, catalysing the first committed step in peptidoglycan biosynthesis, is a target of Clp-dependent proteolysis in Bacillus subtilis[J]. Mol Microbiol, 2004,51(4):1087-102.
[30]	万春云,汪庆,李思敏,等.群体感应对细菌生物膜及细菌耐药性影响的研究进展[J].生态毒理学报,2023,18(1):149-159.
[31]	Wozniak DJ, Tiwari KB, Soufan R, et al. The mcsB gene of the clpC operon is required for stress tolerance and virulence in Staphylococcus aureus[J]. Microbiology, 2012, 158(Pt 10): p.2568–2576
[32]	Tao L, Chattoraj P, Biswas I. CtsR regulation in mcsAB-deficient Gram-positive bacteria[J]. J Bacteriol, 2012, 194(6): p. 1361-1368.
[33]	Jarjec K K. Molecular detection of type III secretory toxins in Pseudomonas aeruginosa isolates[J]. Acta Medica Mediterr,2022,38(2):1363-1370.
[34]	Kajfasz JK, Martinez AR, Rivera-Ramos I, et al. Role of Clp proteins in expression of virulence properties of Streptococcus mutans[J]. J Bacteriol, 2009,191(7):2060-2068.
[35]	Frees D, Chastanet A, Qazi S, et al. Clp ATPases are required for stress tolerance, intracellular replication and biofilm formation in Staphylococcus aureus[J]. Mol Microbiol, 2004,54(5):1445-1462.
[36]	Illigmann A, Thoma Y, Pan S, et al. Contribution of the Clp Protease to Bacterial Survival and Mitochondrial Homoeostasis[J]. Microb Physiol, 2021,31(3):260-279
[37]	Chattoraj P, Banerjee A, Biswas S, et al. ClpP of Streptococcus mutans differentially regulates expression of genomic islands, mutacin production, and antibiotic tolerance[J]. J Bacteriol, 2010,192(5):1312-1323.
[38]	Sun D, Liu Y, Peng X, et al. ClpP protease modulates bacterial growth, stress response, and bacterial virulence in Brucella abortus[J]. Vet Res, 2023,54(1):68.
[39]	Culp E, Wright GD. Bacterial proteases, untapped antimicrobial drug targets[J]. J Antibiot (Tokyo), 2017,70(4):366-377.
[40]	Sassetti E, Durante Cruz C, Tammela P, et al. Identification and Characterization of Approved Drugs and Drug-Like Compounds as Covalent Escherichia coli ClpP Inhibitors[J]. Int J Mol Sci, 2019,20(11):2686.
[41]	Silber N, Pan S, Sch?kermann S, et al. Cell Division Protein FtsZ Is Unfolded for N-Terminal Degradation by Antibiotic-Activated ClpP[J]. mBio, 2020, 11(3): e01006-20.
[42]	雷芳草,阳燕,张剑英.Hsp100/Clp ATPase对变异链球菌毒力表达的调控及机制的研究进展[J].中华口腔医学研究杂志,2018,12(3):190-194.
[43]	Moreno-Cinos C, Goossens K, Salado IG, et al. ClpP Protease, a Promising Antimicrobial Target[J]. Int J Mol Sci, 2019,20(9):2232.

Advances in the effect of ClpP protease on homeostasis and virulence in oral pathogens

Knowledge

Abstract

Cite this article

share this article

References 43

Related Articles 0

Recommended Articles

Metrics

Comments