Oral Biomedicine ›› 2021, Vol. 12 ›› Issue (4): 230-235.

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Effects of epigallocatechin-3-gallate and epigallocatechin-3-O-(3-O-methyl)-gallate containing functional preconditioning liquid on the boding stability of intraradicular dentin

  

  • Received:2021-03-19 Revised:2021-12-14 Online:2021-12-25 Published:2022-01-04
  • Contact: Ling Li E-mail:liling1922003@163.com

Abstract: Objective: To evaluate the effect of epigallocatechin-3-gallate and epigallocatechin-3-O-(3-O-methyl)-gallate containing functional preconditioning liquid on the boding stability of intraradicular dentin, hoping to find a new strategy to improve the stability of intraradicular dentin bonding interface. Methods: EGCG and EGCG-3Me was incorporated into distilled water to acquire the preconditioning liquid with concentration of 200、400 and 600 μg/mL, distilled water was used as Control Group. Build root canal-infection model and observe the antibacterial effect of preconditioning liquid with scanning electron microscope (SEM) and laser scanning confocal microscope (LSCM). After post preparation, acid etching and washing with each group of preconditioning liquid, 84 teeth collected were prepared as fiber post bonding specimens.The push-out bond strength of instant testing and aging with thermocycling for 5,000 times were also tested. Results: The preconditioning liquid showed inhibiting effect on the colonized bacteria in dentinal tubule and performed better with higher concentration. The immediate push-out bond strength showed no significant difference between control group and preconditioning liquid group (P > 0.05). The aging push-out bond strength showed no obvious decrease for preconditioning liquid group (P > 0.05). Conclusions: EGCG and EGCG-3Me preconditioning liquid showed anti-bacterial effect to the colonized bacterial in dentinal tubule and enhanced the bonding stability of intraradicular dentin-adhesive interfaces.

Key words: epigallocatechin-3-gallate, epigallocatechin-3-O-(3-O-methyl)- gallate, anti-bacterial, intraradicular dentin, bonding