TEAD2在口腔鳞癌中的表达及其对细胞生物学行为的影响

Abstract

Abstract: Objective:To investigate the expression pattern of transcriptional enhancement-associated domain 2 (TEAD2) and its effect on cell biological behavior in patients with oral squamous cell carcinoma (OSCC). Methods:We selected 97 OSCC and 20 healthy oral mucosa samples. The expression level of TEAD2 in these samples was detected by immunohistochemical (IHC) staining, and the relationships between its expression and clinical parameters and prognosis of OSCC patients were analyzed. Using small interfering RNA (siRNA) to transfect and silence TEAD2 of CAL27 cells and exploring the effects of TEAD2 silencing on the apoptosis, migration and invasion of CAL27 cells by flow cytometry, wound healing assay and transwell invasion assay. The expression of epithelial-mesenchymal transformation related proteins and genes were respectively detected by Western blot and qRT-PCR. Results:IHC results showed that the expression of TEAD2 in OSCC tissues was significantly higher than that of normal oral mucosa (P<0.05). The high expression of TEAD2 was correlated with tumor size, pathological grade, cervical lymph node metastasis. High expression group of TEAD2 had lower overall/disease-free survival rate of patients (P<0.05). After TEAD2 was silenced by siRNA, the apoptotic ability of CAL27 cells was increased, and the invasion and migration ability were decreased significantly (P<0.01). The protein and mRNA expression level of E-cadherin were significantly upregulated, while the expression level of N-cadherin, Vimentin and Snail were significantly decreased after TEAD2 was silenced (P<0.01). Conclusions:Our findings revealed that TEAD2 was overexpressed in OSCC, its high expression was related to the poor prognosis of OSCC patients, and involved in the regulation of OSCC cell migration, invasion and apoptosis.

Key words: oral squamous cell carcinoma, TEAD2, migration, invasion, apoptosis

CLC Number:

R739.85

References 15

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Expression of TEAD2 and its effect on cell biological behavior in oral squamous cell carcinoma

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