Oral Biomedicine ›› 2022, Vol. 13 ›› Issue (2): 92-95.

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Study of building a co-culture cell sheets of Hertwig’s epithelial root sheath cells and dental follicle cells

1,Wei-Dong TIAN   

  • Received:2022-03-23 Revised:2022-04-27 Online:2022-06-25 Published:2022-07-07
  • Contact: Wei-Dong TIAN E-mail:drtwd@sina.com

Abstract: ?Objective:To build a direct co-culture cell sheets of Hertwig's epithelial root sheath (HERS) cells and dental follicle cells (DFC) by simulating the microenvironment, so as to provide a new strategy for epithelium to participate in the regulation of periodontal ligament/cementum regeneration. Methods:The HERS cells and DFC in the same development stage and the same tooth germ were directly co-cultured to prepare the cell sheets. The morphological and histological characteristics were observed. Then we compared the differences of periodontal ligament/cementum formed abilities between them and dental follicle cell sheets. Results:The composite cell sheets were successfully constructed, which had much cell layers and could secrete abundant extracellular matrix. Compared with DFC sheets, the expressions of mineralization related proteins alkaline phosphatase (ALP) and bone sialoprotein (BSP) were higher. At the same time, the expressions of osteocalcin (OCN) and periodontal ligament associated proteins collagen I (COLI) were positive, while the expressions of OCN and COLI in DFC sheets were negative. Conclusions:The co-culture cell sheets have stronger abilities to form periodontal ligament/cementum. The HERS cells play a crucial regulatory role in the development of periodontal ligament/cementum.

Key words: epithelial root sheath cells, dental follicle cells, cell sheets, tooth regeneration