Top Read Articles

Published in last 1 year |

In last 2 years |

In last 3 years |

All

Please wait a minute...


For Selected: Download Citations EndNote Reference Manager ProCite BibTeX RefWorks Toggle Thumbnails

Select

Research progress on the interaction between Actinobacillus actinomycetemcomitans and human gingival epithelial cells null Oral Biomedicine    2023, 14 (3): 192-196.   Abstract （184）            Knowledge map    Save Actinobacillus actinomycetemcomitans is a gram-negative facultative anaerobe, which is one of the main pathogenic bacteria of periodontitis. With a variety of virulence factors, it can not only damage the barrier structure of gingival epithelial cells and evade the immune defense of the host, but also invade deep tissues and destroy the soft tissue and alveolar bone. The interaction between Actinobacillus actinomycetemcomitans and human gingival epithelial cells is the first step of bacterial invasion of periodontal tissue and the key to the immune response caused by the bacteria. In this paper, we review the studies on adhesion and colonization of human gingival epithelial cells by Actinobacillus actinomycetemcomitans and its interaction with gingival epithelial cells. Reference | Related Articles | Metrics

Select

The mutual relationship and mechanisms between periodontal disease and autoimmune diseases Oral Biomedicine    2025, 16 (1): 1-5.   Abstract （164）            Knowledge map    Save Periodontal disease is a destructive inflammatory condition affecting the supporting tissues of the teeth and is closely associated with rheumatoid arthritis, systemic lupus erythematosus, and other autoimmune diseases, while the precise biological mechanisms underlying this interaction remain unclear. This article aims to provide a detailed overview of the mechanisms behind the interaction between periodontal disease and autoimmune diseases. Related Articles | Metrics

Select

Research progress of physical methods to accelerate orthodontic tooth movement Oral Biomedicine    2023, 14 (3): 141-145.   Abstract （159）            Knowledge map    Save How to accelerate orthodontic tooth movement rates and promote effective bone remodeling, thereby shortening treatment periods and reducing complications, is a difficult and urgent issue in orthodontic clinics. To date, the auxiliary interventions for accelerating orthodontic tooth movement are mainly divided into two categories: surgical interventions and non-surgical interventions. Physical strategies of non-surgical interventions have become the key research direction in this field, with the advantages of non-invasiveness, painlessness, and simplicity of operation. In this paper, we summarize and compare current physical methods of accelerating orthodontic tooth movement. We conclude and outlook the translational applications of current physical methods of accelerating orthodontic tooth movement, providing a reference for achieving ahealthy and efficient orthodontic tooth movement acceleration strategy. Related Articles | Metrics

Select

null Oral Biomedicine    2024, 15 (5): 245-251.   Abstract （158）            Knowledge map    Save The repair of defects in the craniomaxillofacial region presents significant challenges in clinical practice. Various biomaterials for craniomaxillofacial tissue engineering are emerging as potential alternatives to autologous tissue transplantation. By integrating the developmental physiology of craniomaxillofacial tissues with the regenerative mechanisms of injury repair, this article reviews key advancements in the field of bionic regenerative repair of craniomaxillofacial tissue defects, drawing on our research findings. Furthermore, it explores the critical role of bionics in tissue engineering, aiming to provide fresh insights and innovative ideas for the design of future tissue engineering tools and strategies. Related Articles | Metrics

Select

Effect of silencing PGC-1α on the directional differentiation of human stem cells from apical papilla null Oral Biomedicine    2023, 14 (3): 146-150.   Abstract （149）            Knowledge map    Save ObjectiveTo explore the effects of silencing peroxisome-proliferator-activated receptor γ coactivator-1α (PGC-1α)on the directional differentiation of human stem cells from apical papilla (hSCAPs). Methods Expression level of PGC-1α was detected by qRT-PCR after SCAPs were cultured in mineralized induction solution for 0, 3 and 7 days. HSCAPs with low expression of PGC-1α were constructed stably by siRNA transfection, and the knockdown efficiency of PGC-1α was verified by qRT-PCR and Western blot.Further, the effects of silencing PGC-1α on the directional differentiation of hSCAPs were observed by alkaline phosphatase and alizarin red staining and qRT-PCR. Results The expression level of PGC-1α was gradually up-regulated during the directional differentiation of hSCAPs, and hSCAPs with low expression of PGC-1α was successfully constructed. As compared with the control group,the expression level of indicators related with osteo/odontogenic differentiation in hSCAPs silencing PGC-1α were downregulated, mineralized nodules decreased, and the expression of alkaline phosphatase decreased. Conclusion Silencing PGC-1αcan inhibit the directional differentiation capacity of hSCAPs. Related Articles | Metrics

Select

Resveratrol protects bone marrow mesenchymal stem cells by inhibiting ferroptosis null Oral Biomedicine    2023, 14 (3): 162-168.   Abstract （142）            Knowledge map    Save Objective:?To explore the protective effect of Res on BMSCs under oxidative stress in vitro. Methods:? Rat BMSCs were treated with 200 μmol/L hydrogen peroxide to undergo oxidative stress. CCK8, ROS fluorescent probe, qRT-PCR, Western blot and ALP staining examined the effects of different concentration of Res on the cell viability, intracellular ROS levels, osteogenic differentiation and ferroptosis in rat BMSCs under oxidative stress. Results:?Res between 1 and 25 μmol/L promoted the cell viability of rat BMSCs stimulated by hydrogen peroxide (P<0.05). 10 μmol/L Res significantly reduced the ROS levels of hydrogen peroxide -stimulated rat BMSCs and increased the expression of antioxidant-related genes, such as NRF2, CAT, SOD-1 and SOD-2 (P＜0.05). 10 μmol/L Res promoted the expression of osteogenic-related markers of hydrogen peroxide -stimulated rat BMSCs (P＜0.05), significantly enhanced the ALP staining effect, promoted the expression of ferroptosis-related markers (GPX4, XCT) (P＜0.05), decreased the expression of acyl-CoA synthetase long-chain family member(ACSL4), prostaglandin-endoperoxide synthase 2 (PTGS2) and nuclear receptor coactivator 4 (NCOA4) (P＜0.05). Conclusions:?Res could reduce the level of ROS and ferroptosis of oxidative stress-induced rat BMSCs, maintain the cell viability and promote the osteogenic differentiation of rat BMSCs under oxidative stress. Reference | Related Articles | Metrics

Select

A preliminary study of polyvinyl alcohol oral dressing patches loaded with berberine Oral Biomedicine    2025, 16 (1): 14-19.   Abstract （138）            Knowledge map    Save Objective: To investigate the feasibility of a polyvinyl alcohol (PVA) patch loaded with berberine (BBR) for topical application as a postoperative oral dressing. Methods: The PVA patches were placed in aqueous solutions of BBR with different mass fractions (0, 2.5%, 5%), and the PVA patch materials containing different concentrations of BBR were prepared by drying after the BBR was dispersed homogeneously, and they were characterised and tested. The MTT method was used to test the biocompatibility of each group of patches. The antibacterial effects of each group of patch materials on Porphyromonas gingivalis and Fusobacterium nucleatum were compared by the circle of inhibition method. Finally, the haemostatic properties of the patch materials were assessed by in vitro procoagulant assay and rat tail-breaking model. Results: The drug-carrying patch materials in each group had good physicochemical properties and were capable of sustained and stable release of BBR in vitro. The patches in each group showed good biocompatibility. Compared with PVA patches, 2.5% BBR/PVA and 5% BBR/PVA patches showed significant bacteriostatic effects against periodontal pathogens P. gingivalis and F. nucleatum（P<0.001）. In vivo and vitro haemostatic experiments also showed that all groups had good haemostatic effects（P<0.001）. Conclusions: The BBR-loaded PVA patch material has good physicochemical and biological properties, and has good antibacterial effect against common periodontal pathogens and good haemostatic effect, which is expected to be used as a new type of oral topical antibacterial and haemostatic material. Reference | Related Articles | Metrics

Select

Single-retainercantilever resin-bonded bridge in replacing single anterior tooth:A 3 years clinical observation Oral Biomedicine    2023, 14 (4): 253-257.   Abstract （137）            Knowledge map    Save Objective: To observe and analyze the clinical effect of single-retainer cantilever resin-bonded bridge in replacing single anterior tooth missing. Methods: Twenty single-retainer cantilever resin-bonded bridges were fabricated for single anterior tooth missing，and they were evaluated 6，12，24 and 36 months after restoration using the clinical evaluation criteria set by the American Public Health Association (APHA). At the same time, the periodontal indexes of the abutment tooth and the adjacent teeth before and after restoration were recorded to analyze the influence of the restoration on periodontal condition of the abutment teeth, and the VAS of patient satisfaction was quantified. Results: In 20 cases of single retainer cantilever resin-bonded bridge, no debonding or fracture occurred during 36 months of repair period. 1 case was classified as grade B due to edge coloring and the rest were graded. A 24 months after repair; 36 months after the completion of the restoration, 3 cases were classified as grade B due to edge coloring, and the rest were grade A. The patients' satisfaction with the restoration effect was 100%, and there was a significant difference in patients' satisfaction with the status of missing tooth area before and after restoration (P＜0.05). And there was no significant difference in the periodontal indexes of the abutment tooth before and after restoration (P＞0.05). Conclusions: For patients with single anterior tooth missing, using a single retainer cantilever resin-bonded bridge for restoration can obtain a relatively stable restoration results. Related Articles | Metrics

Select

Oral Biomedicine    2023, 14 (4): 263-268.   Abstract （137）            Knowledge map    Save Dental pulp stem cells (DPSCSs) are subject to odontogenic mesenchymal stem cells, which possess the ability to differentiate into odontoblasts and form restorative dentin under certain influence factors. DPSCSs have potential application value for the clinical treatment of pulp diseases and dentine repair. This paper systematically reviewed the influence factors on the odontogenic differentiation of dental pulp stem cells, such as biotic factors, chemical factors, physical factors, gene transfection, bioactive materials and explored the conditions affecting the odontogenic differentiation of dental pulp stem cells. It provides the theoretical basis for the therapies of caries, pulp and periapical diseases and applications in other fields. Related Articles | Metrics

Select

Research progress on the mechanism of action of quercetin in head and neck cancer management null Oral Biomedicine    2023, 14 (3): 206-210.   Abstract （130）            Knowledge map    Save Head and neck cancer is a common malignancy with a high mortality rate and poor prognosis. Quercetin, a flavonoid, is commonly found in many fruits, vegetables, and medicinal herbs and has a variety of biological and pharmacological effects. It has been shown to have anti-tumor effects on a variety of cancers, including head and neck cancer, and can effectively prevent and treat head and neck cancer by inhibiting tumor cell growth, inducing tumor cell apoptosis, inhibiting tumor invasion and metastasis, inhibiting angiogenesis, reversing drug resistance, and enhancing chemotherapy sensitization in a variety of ways. This paper aims to review the mechanism of action of quercetin in treating head and neck cancer in recent years. Reference | Related Articles | Metrics

Select

The comparative study of the coordination of the width between dental arch and basal bone arch in Class Ⅲ and Class Ⅰ malocclusion Oral Biomedicine    2023, 14 (3): 188-191.   Abstract （128）            Knowledge map    Save [Abstract] Objective: To analyze the coordination between the widths of dental and basal bone arches in Class III and Class I malocclusion using cone-beam CT (CBCT). Methods: Sixty patients (18-30 y), including 30 cases each of Class III and Class I, were selected to collect CBCT scans and perform three-dimensional imaging to measure the dental arch widths of the maxillary and mandibular cuspid, premolar and molar regions and the corresponding basal bone arch widths. the differences between the dental arch widths in the same tooth sites and the basal bone arch widths were calculated. The difference between the width of the dental arch and the basal bone arch was compared between Class III and Class I malocclusion. Results: The dental arch width of the first maxillary molar was significantly greater in Class III than in Class I (P<0.05), and there was no difference between the dental arch widths of the maxillary cuspids and the first premolars(P>0.05); the dental arch widths of the mandibular cuspids and the first molars were significantly greater in Class III than in Class I (P<0.05), and there was no difference between the dental arch widths of the mandibular first molars(P>0.05). The basal bone arch width of the maxillary first premolars was significantly smaller in Class III than in Class I(P<0.05), and the basal bone arch width of the maxillary cuspids and first molars were not different (P>0.05);the basal bone arch widths of all the mandibular tooth sites were significantly greater in Class III than in Class I(P<0.05). In the maxilla, except for the cuspid site, the difference between the dental arch and the basal bone arch widths in Class III was significantly greater than in Class I (P<0.05); in the mandible, except for the first molar site, the difference between the dental arch and the basal bone arch widths in Class III was significantly smaller than in Class I (P<0.05). Conclusions: By comparing the results of the difference between the dental arch and the basal bone arch widths in Class III and Class I malocclusion, during the treatment of Class III malocclusion, we can increase the maxillary basal bone arch width in the posterior region to improve the coordination of the dental arch and the basal bone arch widhs, and increase the mandibular dental arch width in the posterior region to improve the coordination of the dental arch and the basal bone arch widths. Reference | Related Articles | Metrics

Select

Macrophage MVP regulates bone regeneration by promoting BMSC osteogenic differentiation Yan YANG Na ZHAO Oral Biomedicine    2023, 14 (3): 151-155.   Abstract （126）            Knowledge map    Save Objective:?To investigate the role of macrophage major vault protein (MVP) on bone regeneration. Methods:? Macrophage-specific MVP knockout (CKO) and wild-type (WT) C57BL/6 mice were used to establish a tibial mono-cortical defect model, and the bone regeneration was observed by Micro-CT and hematoxylin-eosin (H&E) staining. Macrophages from WT or CKO mice bone marrow were co-cultured with wild-type bone marrow mesenchymal stem cell (BMSC), respectively. The effect of macrophage MVP on BMSC osteogenic differentiation was tested by alkaline phosphatase (ALP) staining and qRT-PCR. Finally, a cytokine array was used to test the cytokine profile of macrophages to understand the regulation of MVP on cytokines secretion. Results:?On day 14 of bone defect, Micro-CT scan and reconstruction results showed that the mineralized tissue in the defect area of CKO mice was less than that of WT mice (P＜0.05); hematoxylin-eosin staining results showed that the bone area in the defect area of CKO mice was less than that of WT mice; In the in vitro co-culture assay, the levels of osteogenic differentiation marker genes were significantly lower in BMSC co-cultured with CKO macrophages than in those co-cultured with WT macrophages (P＜0.05). Cytokine array results showed that macrophages lacking MVP secreted more cytokines including CCL2, CCL4, CXCL10, CCL3, IL-7 that inhibit osteogenic differentiation. Conclusions:?Macrophage MVP may regulate bone regeneration by promoting BMSC osteogenic differentiation. Reference | Related Articles | Metrics

Select

Preliminary study on preparation and therapeutic effect of Lianbu sustained-release gel for experimental periodontitis Oral Biomedicine    2023, 14 (4): 216-223.   Abstract （126）            Knowledge map    Save Objective:Prepare Lianbu sustained-release gel, to examine its basic physicochemical properties, degree of release and toxicological properties, and to evaluate its pharmacodynamic effects in the treatment of experimental periodontitis. Methods:The prescription process for the preparation of Lianbu sustained-release gel was optimized by alkaline phosphatase assay to obtain the optimal mass ratio of the extract of coptis and the extract of rhizome drynariae. Glycerol, glyceryl triacetate, hydroxyethyl cellulose and polyacrylic acid resin were used as gel matrix to prepare Lianbu sustained-release gel with 2%, 4%, and 6% drug-loading rate, and their morphology, pH, stability, and the release rate in vitro were examined to optimize the optimal amount of drug loading. Acute toxicity test in mice at 20 g/kg by gavage using the maximum tolerated dose method. The mucosa of the lower lip of SD rats was sutured into a blind pouch, and then the sustained-release gel was injected into the pouch and applied to their lower gingival mucosa for local mucosal irritation experiments. Long-term toxicology experiments were conducted in SD rats by continuous gavage for 90 days to examine the blood routine and histopathological indexes. An experimental periodontitis model was established in SD rats, and the three-dimensional morphology of the alveolar bone of rats was observed using micro-CT, and the CEJ-ABC was measured. Results:The optimum mass ratio for the preparation of coptis extract and rhizome drynariae extract was 1∶10. The 2%, 4%, and 6% Lianbu sustained-release gel all had good injectability, were in the neutral pH range, and were well stabilized. 6% Lianbu sustained-release gel had the best effect on release. No acute toxic side effects were seen in mice given the sustained-release gel. The mucous membrane contact test did not show any significant irritant reactions such as erythema and edema. Long-term toxicology experiments showed no difference in general activity status, behavioral activities, diet, urine and stool, weight gain, hematology of rats in the Lianbu sustained-release gel group compared with the control group, and no obvious pathological changes were seen in the pathological examination of organs, indicating that the drug had no obvious toxic side effects. After treating experimental periodontitis rats for 4 weeks, micro-CT imaging results showed the alveolar bone resorption was significantly improved in 6% gel group and CEJ-ABC was significantly less than that in blank gel group and control group（P<0.05）. Conclusions:The prepared 6% Lianbu sustained-release gel had excellent physicochemical properties and slow-release performance, and had certain therapeutic effects on experimental periodontitis rats. Related Articles | Metrics

Select

Stress analysis of pterygomaxillary implant design in implant restoration of maxillary posterior partial edentulism Oral Biomedicine    2023, 14 (3): 175-182.   Abstract （124）            Knowledge map    Save Objective：To study the stress distribution after pterygomaxillary implantation in posterior maxillary alveolar by using three-dimensional finite element method. Methods：In six models with dentition defects in the posterior maxillary region, tilted implants were placed at 45° to occlusal plane (45° tilted implantation) or at 70° to Frankfort plane (70° tilted implantation) and constructed fixed bridges with normal implants in the premolar maxillary region. The static force of 200N was vertically loaded on the second molar or first molar of the fixed bridges to analyze the stress distribution in the posterior maxillary alveolar bone. Results：①The stress of alveolar bone was 43.18 17.15MPa after 200N vertical force loading to 70° tilted implantation while it was 67.25 18.44MPa in 45° tilted implantation. ② The stress of alveolar bone was about 32.49 19.01MPa when 200N vertical force loading to the second molar of fixed bridges constructed with the 70° tilted implant and normal implant while the stress was about 26.89 14.66MPa when 200N vertical force loading to the first molar of the fixed bridges. ③ The stress of alveolar bone was about 39.88 17.12MPa when 200N vertical force loading to the second molar of fixed bridges constructed with the 70° tilted implant and normal implant while the stress was about 31.03 15.05MPa when 200N vertical force loading to the first molar of the fixed bridges. Conclusion: The pterygomaxillary implantation was applied to repair dentition defects in the maxillary posterior region, 70° tilted implantation with the single crown or fixed bridge could be selected according to the abutment puncture point. The 45° tilted implantation should be combined with mesial implants or natural teeth to form fixed bridge to avoid biological complications caused by the stress exceeding the limit of alveolar bone tissue. Reference | Related Articles | Metrics

Select

Expression of CKS2 and its effect on cell biological behavior in oral squamous cell carcinoma Oral Biomedicine    2023, 14 (4): 233-238.   Abstract （117）            Knowledge map    Save Objective: To investigate the expression pattern of cyclin-dependent kinase regulatory subunit 2 (CKS2) and its effects on cell biological behavior in patients with oral squamous cell carcinoma (OSCC). Methods: Bioinformatics analysis was conducted to explore the expression level of CKS2 mRNA in OSCC and normal mucosal tissues. We selected 91 OSCC and 20 healthy oral mucosa samples. Immunohistochemical (IHC) staining was used to detect the expression level of CKS2 in 91 specimens of OSCC patients and 20 normal oral epithelial mucosa, and to explore the predictive value of CKS2 expression in OSCC patients. Small interfering RNA (siRNA) was used to silence CKS2 of Cal27 cells and its knockdown efficiency was determined by western blot. The effects of CKS2 silencing on the proliferation, cycle and migration of Cal27 cells were detected by cell clone formation assay, cycle assay and wound healing assay. Results: Bioinformatics analysis and IHC results showed that the expression of CKS2 in OSCC tissues was significantly higher than that of normal oral mucosa (P＜0.05). The expression of CKS2 was correlated with tumor size, cervical lymph node metastasis and survival rate of patients (P＜0.05). CKS2 silencing inhibited the proliferation and migration of Cal27 cells and induced G2 phase arrest (P＜0.05). Conclusions: Our findings revealed that CKS2 was overexpressed in OSCC, its high expression relating to the poor prognosis of OSCC patients, and involving in the positive regulation of OSCC cell proliferation, migration and cycle. Reference | Related Articles | Metrics

Select

The effect of connective tissue growth factor on NELL-1-induced osteogenesis in MC3T3-E1 cells null Oral Biomedicine    2023, 14 (3): 169-174.   Abstract （109）            Knowledge map    Save Objective: This study aims at exploring the role of connective tissue growth factor (CTGF) on Nel-1 like molecule type 1(NELL-1)-induced osteogenesis in MC3T3-E1 cells. Methods: The MC3T3-E1 cell lineage was used in the study, which was treated with each of control (PBS), NELL-1 alone, CTGF alone or in combination of NELL-1 and CTGF. Then cell proliferation was employed with help of CCK-8 assay. After osteogenic induction, multiple experimental techniques including alizarin red staining with quantitative analysis, ALP activity assay and ALP staining, quantitative real time poly chain reaction (qRT-PCR) and western blot were used to detect cell osteogenic differentiation among each group. Results: Compared to control, CTGF partly inhibited MC3T3-E1 cell proliferation; while NELL-1 treatment alone induced more mineralized nodules formation, promoted ALP activity and darker ALP staining in MC3T3-E1 cells with increased transcription and expression levels of osteogenic genes, such as RUNX2, ALP and OSX (P<0.01). However, compared to NELL-1 treatment alone, less mineralized nodules formation, decreased ALP activity and light ALP staining with dramatically down-regulated transcription and expression of RUNX2, ALP and OSX were found in cells treated with in combination of NELL-1 and CTGF (P<0.05). Conclusions: CTGF inhibited NELL-1-induced osteogenesis in MC3T3-E1 cells.s.?   Reference | Related Articles | Metrics

Select

Study on the Role of c-Jun N-terminal Kinase/Nuclear Factor-κB/Caspase-3 in Renal Cell Apoptosis Induced by Experimental Periodontitis in Mice. Oral Biomedicine    2025, 16 (1): 26-33.   Abstract （108）            Knowledge map    Save Objective:?To investigate the role of c-Jun N-terminal kinase (JNK)/nuclear factor-κB (NF-κB)/Caspase-3 in kidney dysfunction related to periodontitis by establishing a mouse model of experimental periodontitis. Methods:?Twelve SPF-grade male C57 mice were randomly divided into a control group and a periodontitis group. Periodontitis was induced by ligating silk around the necks of the bilateral maxillary second molars. Four weeks later, the mice were sacrificed, and the three-dimensional structure of the alveolar bone was reconstructed using Micro-CT to analyze bone resorption. Histological changes in periodontal and kidney tissues were observed using hematoxylin-eosin (HE) and periodic acid-schiff (PAS) staining. Biochemical kits were used to detect kidney oxidative stress biomarkers malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), and kidney function indicators creatinine (Cre) and albumin (Alb). Mitochondrial superoxide red fluorescent probe staining was employed to assess ROS levels in kidney tissues, while terminal dexynucleotidyltransferase-mediated dUTPnick end labeling (TUNEL) staining evaluated renal cell apoptosis. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure expression levels of NF-κB, interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), B-cell lymphoma 2 (Bcl-2), and in B-cell lymphoma-2-associated X protein (Bax) kidney tissues. Immunohistochemistry was performed to detect phosphorylated JNK (p-JNK), JNK, NF-κB, and Caspase-3 expressions in kidney tissues. Results: Compared to the control group, mice in the periodontitis group exhibited gingival hyperemia and swelling around the maxillary second molars. Micro-CT analysis revealed significant alveolar bone resorption in the periodontitis group, with an increased distance from the enamel-dentin junction to the alveolar ridge crest. Iirregular elongation of the gingival epithelial rete pegs, accompanied by resorption at the alveolar ridge crest, damage to the glomerular structure, and expansion of Bowman's space were showed. The thickening of the glomerular basement membrane and a reduction in the brush border of renal tubules in the periodontitis group were indicated. Biochemical assays demonstrated elevated MDA levels, decreased SOD and GSH levels in the kidney tissue of periodontitis mice (P＜0.05), along with increased serum levels of Cre and Alb (P＜0.05). Elevated ROS levels in the renal tissue of periodontitis mice was shown(P＜0.05). TUNEL staining results indicated an increase in the number of apoptotic renal cells in the periodontitis group (P＜0.05). qRT-PCR and immunohistochemistry showed that the expressions of IL-6, TNF-α, Bax, and NF-κB in the kidney tissue of periodontitis mice were increased (P＜0.05), while the expression of Bcl-2 was decreased (P＜0.05). The expression of JNK, p-JNK, NF-κB, and Caspase-3 was significantly elevated (P＜0.05). Conclusions: The JNK/NF-κB/Caspase-3 may play a crucial role in affecting renal cell apoptosis, contributing to kidney dysfunction induced by periodontitis. Reference | Related Articles | Metrics

Select

Effects of estrogen deficiency on the proliferation and odonto/osteogenic differentiation of rat stem cells from the apical papilla null Oral Biomedicine    2023, 14 (3): 156-161.   Abstract （107）            Knowledge map    Save Objective：To evaluate the effects of estrogen deficiency on the proliferation and odonto/osteogenic differentiation of rat stem cells from the apical papilla(SCAPs). Methods: Ten 8-week-old female SD rats were randomly divided into two groups, sham-operation group(Sham) and ovariectomized group(OVX), 5 rats in each group. All rats were anesthetized and both ovaries of OVX-rats were gently removed. Sham-operation rats were treated with the same incisions to expose the ovaries but without any hurt to them. One month after the operation, the apical papillae of rat incisors were gently separated and SCAPs were isolated and incubated. Methyl thiazolyl tetrazolium(MTT) assay, alkaline phosphotase(ALP) activity, real-time reverse transcription（RT-PCR）and western blot assay were respectively used to determine the proliferation and odonto/osteogenic differentiation potential of rat SCAPs. Cell pellets of OVX-SCAPs and Sham-SCAPs were respectively transplanted into the renal capsules of immunodeficient female rats. After in vivo culture for 8 weeks, the retrieved implants were processed for hematoxylin and eosin (H & E) staining. Results: MTT results showed that OVX-SCAPs exhibited the decreased proliferative activity as compared with Sham- SCAPs. ALP activity of OVX- SCAPs was lower than that of Sham- SCAPs (P<0.01). Real-time RT-PCR and Western blot results showed that the expression of odonto/osteogenic markers (Dspp/DSP, Osx/OSX, Ocn/OCN) in OVX- SCAPs was significantly inhibited as compared with Sham- SCAPs (P<0.01). Sham-SCAPs implants showed the typical dentin-pulp like complex, while the retrieved implants of OVX-SCAPs exhibited unregular tissue. Conclusions: Estrogen deficiency significantly inhibits the proliferation and odonto/osteogenetic potential of rat SCAPs. Related Articles | Metrics

Select

Study on the Role of ENSR00000313345 in the Tumorigenesis and Development of Head and Neck Squamous Cell Carcinoma null Oral Biomedicine    2024, 15 (5): 257-264.   Abstract （106）            Knowledge map    Save Objective:?To investigate the clinical effect of artificial intelligence designed zirconia full crown and traditional CAD/CAM designed zirconia full crown in repairing molar defects. Methods:?A total of 116 cases of molars after root canal treatment in our hospital were selected as the research objects from March 2022 to April 2023. By using random number table method, they were randomly divided into the study group (zirconia full crown restoration designed by artificial intelligence) and the control group (zirconia full crown restoration designed by traditional CAD/CAM) with 58 cases each. The edge fracture and retention, edge adaptability, adjacent anatomical morphology, periodontal, peripheral mucosa and occlusal adaptability were scored for all patients after prosthesis trial，immediately retention and 1 year restoration. The time required for the restoration designs and total patients' chair-side visit in both groups were recorded and averaged. The satisfaction of patients was investigated to compare the repair treatment effect of the two groups. Results:According to the FDI evaluation criteria, the clinical repair effect of the restorations was evaluated. The shape of crown abduction space designed by AI in the experimental group was more similar to that of natural teeth, while the traditional CAD/CAM designed crown abduction space in the control group lacked natural neck narrowing. The anatomical morphology score of the experimental group was significantly lower than that of the control group, and the difference was statistically significant (P < 0.05), indicating that the AI anatomical morphology design was superior to the traditional CAD/CAM design. After adjustment, there were no statistical differences of two groups in the immediate retention of the restorations and the review one year later (P> 0.05). There were no significant differences between experimental and control groups in other indexes (marginal fracture and retention, marginal adaptability, periodontal, peripheral mucosa and occlusal adaptability) after prosthesis trial，immediately retention and 1 year restoration (P > 0.05). The total time of prosthesis design and the total time of patients' chair-side visit in the experimental group were lower than those in the experimental group, and the differences were statistically significant (P < 0.05). After one year of repair, the average satisfaction of the study group was 95.55%, and that of the control group was 94.83%, the difference was not statistically significant (P> 0.05).Conclusions:Compared with the traditional CAD/CAM design, the crowns designed by AI have no obvious defects in the restoration of molar defects, which can save time and cost, and is suitable for clinical application. Reference | Related Articles | Metrics

Select

Oral Biomedicine    2025, 16 (1): 6-13.   Abstract （106）            Knowledge map    Save Objective: To investigate the effect of Porphyromonas gingivalis(P.g) outer membrane vesicles (OMVs) on bone marrow mesenchymal stem cells (BMSCs) osteogenesis through arginine specific gingipain B (RgpB). Methods: P.g OMVs were isolated and identified, co-cultured with BMSCs. The changes in the gene and protein levels of alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP2), and osteocalcin (OCN) were detected by real-time fluorescent quantitative PCR (qRT-PCR) and Western blot. To investigate the effect of proteins in P.g OMVs on BMSCs osteogenic differentiation, P.g OMVs were treated with proteinase K to remove their protein components and then co-cultured with BMSCs, and Western blot, ALP staining, and alizarin red staining were used to examine changes in osteogenic ability. The results verified whether P.g OMVs inhibit BMSCs osteogenesis through the proteins they carry. Further, liquid chromatography mass spectrometry analysis of P.g OMVs was conducted to screen for potential protein molecules, RgpB. The effect of RgpB on the osteogenic differentiation ability of BMSCs was verified by qRT-PCR and ALP staining.Results:Both qRT-PCR and Western blot results showed that P.g OMVs inhibited the expression of ALP, BMP2, and OCN genes and proteins in BMSCs. When the protein molecules in P.g OMVs were removed, the expression of ALP and BMP2 proteins in BMSCs increased, along with enhanced ALP activity and mineralized nodule formation. Mass spectrometry analysis revealed high expression of RgpB in the OMVs. When BMSCs were stimulated with RgpB protein, the expression of ALP, BMP2, and OCN genes in BMSCs decreased as RgpB concentration increased (P＜0.05), and ALP activity was suppressed. Conclusion: P.g OMVs can inhibit the osteogenic differentiation of BMSCs by carrying the RgpB. Related Articles | Metrics