Abstract: Objective: To identify the effects of dentin non-collagenous proteins (dNCPs) on the proliferation and mineralization of human dental pulp stem cells (hDPSCs) in vitro. Methods: hDPSCs were treated with 10 μg/mL dNCPs in normal medium for certain time periods. Proliferation of hDPSCs was evaluated by MTT colorimetric assay. Alkalinephosphotase (ALP) activity was determined by enzymekinetics methods. Alizarin red staining and cetylpyridinium chloride (CPC) assay were performed to investigate the mineralization ability of hDPSCs. Results: There was no difference between the proliferation activity of hDPSCs treated with dNCPs and the other group (P>0.05). ALP activity and mineralization ability of this group were signigicantly upregulated (P<0.01). Conclusions: 10 μg/mL dNCPs significantly promotes the mineralization of hDPSCs, while the proliferation ability shows no significant difference.