Objective:?To explore the effect of TOM40 on mitochondria respiration of dental pulp fibroblast in the inflammatory microenvironment. Methods: SD rats’ mandibular first molar were used to construct the pulpitis model. Hematoxylin-eosin was used to observe the inflammatory infiltration of pulpitis. Immunohistochemical staining was used to visualize the expression of pro-inflammatory factor IL-1β, TNF-α and 8-OHDG in dental pulp tissue. The apoptosis of dental pulp tissue was detected by TUNEL. Human dental pulp fibroblasts (hDPFs) were cultured in vitro and stimulated with LPS at concentrations of 1, 5 and 10 μg/mL, respectively. The expressions of pro-inflammatory cytokines IL-1β, TNF-α and pro-apoptotic factors Bax and Bak were detected by real-time fluorescence quantitative PCR and Western blot, and the changes of mitochondrial respiratory function of hDPFs were detected by TMRE, DCFH-DA probe and ATP content detection kit. The expression of down-regulated subunits in TOM complex was screened by real-time fluorescence quantitative PCR and verified by immunofluorescence staining. TOM40 overexpression hDPFs cell line was constructed based on the screening results, and LPS stimulation was added to detect the changes of mitochondrial respiratory function. Results：In rats’ molar pulpitis model, with the prolongation of exposure time, the necrotic area of dental pulp, the expression of IL-1β, TNF-α, 8-OHDG and apoptosis were increased（P＜0.05）. In the inflammatory hDPFs model, the expression of IL-1β, TNF-α, Bax and Bak were increased, the respiratory function of mitochondria was impaired, and the expression of TOM40 was specifically down-regulated（P＜0.05）. Overexpression of TOM40 could effectively improve the decrease of mitochondrial membrane potential, ROS accumulation and ATP content of hDPFs induced by LPS stimulation（P＜0.05）. Conclusions：The development of pulpitis was accompanied by oxidative stress and mitochondrial respiratory dysfunction, mitochondrial impaired respiratory function could be saved by overexpress TOM40.

Objective:?This article is to make a comprehensive analysis of oral mucosal wound healing process at the genetic level. Methods:?In this study, a mouse palatal mucosal wound model was constructed by exfoliating the whole layer of soft and hard palatal mucosal tissues from the first to the third molar in the upper jaw of mice, and by extracting the mucosal wound tissues at 0, 1, 3, 5, and 7 days post-traumatization and sequencing the transcriptome RNA, we constructed the characteristics of the gene expression profiles of the various stages of the oral mucosal wound healing through the analysis of the data. Results: Differential genes on days 1, 3, 5, and 7 of mucosal wound healing were compared with those of normal maxillary mucosa and analyzed by GO enrichment. The immune response was active on day 1 of mucosal wound healing (P<0.05), wound healing and peptidases and endopeptidases-related pathways were significantly up-regulated on day 3 (P<0.05), immune response and collagen-containing extracellular matrix-related pathways were significantly up-regulated on day 5 (P<0.05), and expression of genes related to the immune response pathway and collagen-containing extracellular matrix-related genes was active on day 7 (P<0.05). Conclusions：The inflammatory phase of mucosal wound healing is dominated by leukocyte- and immunoglobulin-mediated immune responses during the first three days of wound healing, re-epithelialization begins on the third day of wound healing, and leukocyte- and immunoglobulin-mediated immune responses are active again on the fifth day and beyond, and the expression of collagen-containing components of the extracellular matrix upregulated in the healing of wounds may be associated with mucosal wound healing.

[Abstract] Objective:To investigate the effects of constitutive activation of β-catenin on the initial alveolar bone remodeling after tooth extraction. Methods: Catnblox(ex3)/+mice (control group) and DMP1-9.6kb-cre+/-;Catnblox(ex3)/+mice (experimental group) were constructed. The right maxillary first molar was extracted and were collected 1 week later. Histological stainings were performed to observe the healing of the extraction sockets. Results: Compared with the control group, TRAP staining of the experimental group showed a decrease in the number of osteoclasts (P<0.001), and Masson staining showed decreased collagen mineralization (P<0.01). Immunohistochemistry showed decreased specific staining for osteogenesis-related molecules OCN, DMP1, and OPG (P<0.01). Conclusion: Constitutive activation of β-catenin in DMP1-9.6kb-cre delays the initial healing rate of extraction sockets. [Key words] Extraction socket healing; Alveolar bone remodeling; β-catenin; Wnt/β-catenin signaling pathway;

Objective:?To investigate the effect of graphene oxide, chitosan-modified barium titanate nanoparticles (BCG-NPs) and polyvinylidene fluoride trifluoroethylene (P(VDF-TrFE)) composite piezoelectric materials on the osteogenic differentiation of hWJ-MSC. Methods:?The BCG-NPs/P(VDF-TrFE) (BCGP-Ti) coating was prepared on the surface of titanium by drop coating method. The surface morphology, phase composition, hydrophilicity and piezoelectric properties of the coating were characterized. The ability of the samples to induce mineralization in vitro was tested by simulated body fluid immersion test. hWJ-MSC was inoculated on the surface of the sample, and the cell viability was detected by CCK-8. The adhesion of hWJ-MSC was observed through cytoskeleton staining. ALP and alizarin red staining were used to evaluate ALP activity and mineralization level. The expressions of osteogenic differentiation related genes and proteins were detected through RT-qPCR and immunofluorescence staining. The Ti rods and BCGP-Ti rods were implanted into the femur of SD rats, respectively. Micro-CT and histological staining were applied to examine the osseointegration. Results:?The fabrication of the BCGP-Ti coating was confirmed by scanning electron microscope and X-ray diffraction analysis. After polarization, BCGP-Ti showed good piezoelectric response and better hydrophilicity than that of Ti and unpolarized BCGP-Ti (P<0.01), which induced better mineralization in vitro than those of the other two groups. After polarization, the area of hWJ-MSC spreading on BCGP-Ti surface was higher than that on Ti surface (P<0.05). Compared with Ti, polarized BCGP-Ti not only promoted the expression of osteogenic differentiation related genes including ALP, COLI, RUNX2, OCN and proteins, but also achieved better osseointegration in vivo. Conclusions:?BCG-NPs/P (VDF-TrFE) composite coating on the surface of Ti could promote osseointegration by inducing osteogenic differentiation of hWJ-MSC.

Objective: This study investigated the osteogenic efficacy of phosphorylated polyamideamine (P-PAMAM) dendritic macromolecules crosslinked to collagen scaffolds in SD rats. Methods：Fourier transform infrared spectroscopy （FTIR） was used to characterize the phosphorylation of PAMAM and the morphological identification of three groups of collagen scaffolds, namely collagen scaffold group, PAMAM composite scaffold group and P-PAMAM composite scaffold group. Micro-CT threedimensional reconstruction was used to analyze the changes of bone mass during bone healing after skull defect implantation in rats with composite scaffolds. HE staining and Masson staining were used to analyze the skull specimens of rats. Results：FTIR showed that PAMAM was successfully phosphorylated; scanning electron microscopy showed that the composite scaffold pore formation was good; and Micro-CT analysis of bone volume, ratio of new bone mass to total defect volume, bone mineral density and trabecular bone count, HE staining and Masson staining showed that the P-PAMAM composite collagen scaffold had better osteogenic induction effect than the collagen scaffold and PAMAM composite scaffold. Conclusions：P-PAMAM dendritic macromolecule composite collagen scaffold has a good effect on osteogenesis in vivo.

Objective:?To identify the role of hsa_circ_0030042 in oral squamous cell carcinoma (OSCC). Methods:?The expression of hsa_circ_0030042 in OSCC cells was detected by real time RT-PCR. Transfection of small interfering RNA (siRNA) was performed in OSCC cells to knock down hsa_circ_0030042. The effect of hsa_circ_0030042 on OSCC was validated using CCK-8 assay, colony formation assay, cell scratch assay and Transwell assay. Results:?The expression of hsa_circ_0030042 in CAL27 and HSC3 cells was significantly increased (P＜0.001). Compared with the control group, the proliferation of CAL27 and HSC3 cells was significantly decreased after knockdown of hsa_circ_0030042 (P＜0.05). The migration and invasion ability of CAL27 and HSC3cells were both significantly decreased (P＜0.05). Conclusions:?Hsa_circ_0030042 is significantly upregulated in OSCC and can promote proliferation, invasion and migration in OSCC.

Fatty acids are widely found in living organisms and are important nutrients and metabolites in living organisms, as well as one of the main energy sources of the organism. Fatty acid metabolism is closely related to bone remodeling and the development of osteoporosis, and its metabolic disorder may be one of the pathogenic mechanisms of osteoporosis. Therefore, we review the progress of research on the role of fatty acid metabolism in bone remodeling and osteoporosis.

Periodontitis was defined as chronic inflammatory disease in periodontal tissues initialed by dental bacterial plaque. And there were peripheral nerve terminals extensively distributed in periodontium, including autonomic nerve terminals and sensory nerve terminals. Recent evidences indicated that periodontal peripheral nerves played a significant role in periodontitis, and regulating alveolar bone remodeling and periodontal immune microenvironment. This article reviewed the periodontal distribution of peripheral nerves and the role of peripheral nerves in periodontitis.

Self-assembling peptides, characterized by specific amino acid sequences and structures, can harness internal free energy under certain conditions to spontaneously assemble into nanostructures with enhanced physicochemical stability. This technology has been widely applied in the fields of tissue engineering, cancer therapy, and antibacterial applications, and it has demonstrated significant potential in the repair of dental tissues. This review summarizes the assembly mechanisms, morphologies, and applications of self-assembling peptides in dental tissue repair, discusses key points in peptide design, and outlines the challenges and future directions in this field.

Schwann cells show strong plasticity in Wallerian degeneration after peripheral nerve injury (PNI). They activate into repair schwann cells to clear myelin debris and guide axon regeneration and other important repair processes. Stem cells have the characteristics of self-renewal, multipotent differentiation and immune system regulation, so the therapeutic strategy of using stem cells to induce directional differentiation into this kind of repair schwann cells shows great potential. Odontogenic stem cells (DSCs) have obvious advantages over other stem cells, such as easy access, minimally invasive and negligible ethical issues. This article reviews the directional differentiation, secretion of nutritional factors and gene regulation of various types of odontogenic stem cells in the process of peripheral nerve injury and regeneration in recent years.