Abstract: Objective:?This article is to make a comprehensive analysis of oral mucosal wound healing process at the genetic level. Methods:?In this study, a mouse palatal mucosal wound model was constructed by exfoliating the whole layer of soft and hard palatal mucosal tissues from the first to the third molar in the upper jaw of mice, and by extracting the mucosal wound tissues at 0, 1, 3, 5, and 7 days post-traumatization and sequencing the transcriptome RNA, we constructed the characteristics of the gene expression profiles of the various stages of the oral mucosal wound healing through the analysis of the data. Results: Differential genes on days 1, 3, 5, and 7 of mucosal wound healing were compared with those of normal maxillary mucosa and analyzed by GO enrichment. The immune response was active on day 1 of mucosal wound healing (P<0.05), wound healing and peptidases and endopeptidases-related pathways were significantly up-regulated on day 3 (P<0.05), immune response and collagen-containing extracellular matrix-related pathways were significantly up-regulated on day 5 (P<0.05), and expression of genes related to the immune response pathway and collagen-containing extracellular matrix-related genes was active on day 7 (P<0.05). Conclusions：The inflammatory phase of mucosal wound healing is dominated by leukocyte- and immunoglobulin-mediated immune responses during the first three days of wound healing, re-epithelialization begins on the third day of wound healing, and leukocyte- and immunoglobulin-mediated immune responses are active again on the fifth day and beyond, and the expression of collagen-containing components of the extracellular matrix upregulated in the healing of wounds may be associated with mucosal wound healing.

Key words: [Key words] Oral mucosa, Wound healing, Transcriptome sequencing