牙周局部炎症对小鼠正畸牙齿移动影响的实验研究

›› 2018, Vol. 9 ›› Issue (4): 177-181.

牙周局部炎症对小鼠正畸牙齿移动影响的实验研究

张馨艺¹,陈楠²,李萌²,隋秉东²,马灿灿³,胡成虎²,金钫²

1. 首都医科大学附属北京口腔医院
2. 第四军医大学组织工程中心
3. 邹城市口腔医院

收稿日期:2018-09-03 修回日期:2018-11-28 出版日期:2018-12-25 发布日期:2019-01-07
通讯作者: 金钫 E-mail:fangjin@fmmu.edu.cn
基金资助:
国家自然科学基金

Effect of local periodontitis on orthodontic tooth movement in mice

1. School of Stomatology, Capital Medical University
2. Center for Tissue Engineering, Fourth Military Medical University
3. Stomatological Hospital of Zoucheng

Received:2018-09-03 Revised:2018-11-28 Online:2018-12-25 Published:2019-01-07

摘要/Abstract

摘要： 目的：利用小鼠正畸牙模型探究牙周炎静止期局部炎症对正畸牙齿移动的影响。方法：取12周龄C57小鼠，上颌磨牙区腭侧牙龈局部注射脂多糖（lipopolysaccharide，LPS）建立牙周炎症模型，等量生理盐水注射为对照，micro-CT扫描并定量分析牙槽骨嵴丢失情况。于牙周炎静止期建立小鼠正畸牙齿移动模型，使用30 g力值加力7 d，micro-CT扫描并定量分析上颌骨骨密度和牙移动距离。结果：LPS刺激小鼠所致的牙周炎在静止期进行正畸加力，相比于生理盐水注射后正畸加力，上颌骨骨密度轻度降低（P<0.05），牙移动距离明显增加（P<0.05）。结论：牙周局部炎症环境可促进小鼠正畸牙齿移动。本研究结果为临床牙周—正畸联合治疗中的正畸效果和风险评估提供参考价值，对牙齿移动条件的优化具有一定的指导意义。

Abstract: Objective： To investigate the effect of local periodontitis on orthodontic tooth movement in a mouse model. Methods: 12-week-old C57 mice were enrolled, and injected with lipopolysaccharide (LPS) via the gingiva lateral to the maxillary molar region to establish a model of periodontitis. Mice injected with the same volume of saline were included into the control group. Alveolar bone loss was scanned and quantitatively analyzed using Micro-CT. A mouse model of orthodontic tooth movement was established during the stationary phase of periodontitis. The density of maxillary bone and the distance of tooth movement were scanned and quantitatively analyzed using Micro-CT with the force value set at 30 g at a duration of 7 days. Results：The orthodontic tooth movement during the stationary phase of LPS-induced periodontitis demonstrated a slight decrease in the bone mineral density of maxillary bone (P < 0.05), and a remarkable increase in the distance of tooth movement (P < 0.05) when compared to that after saline injection. Conclusions: Local periodontitiscan facilitate orthodontic tooth movement in mice. Our findings will provide references for the effect and risk assessment of tooth movement in the combined therapy of periodontics and orthodontics in clinical practice, and provide guidance for the optimization of orthodontic tooth movement.

张馨艺陈楠李萌隋秉东马灿灿胡成虎金钫. 牙周局部炎症对小鼠正畸牙齿移动影响的实验研究[J]. , 2018, 9(4): 177-181.

参考文献

[1]Cao H, Kou X, Yang R, et al.Force-induced Adrb2 in periodontal ligament cells promotes tooth movement[J].J Dent Res, 2014, 93(11):1163-1169 [2]Chen N, Sui BD, Hu CH, et al.microRNA-21 Contributes to Orthodontic Tooth Movement[J].J Dent Res, 2016, 95(12):1425-1433 [3]Yan Y, Liu F, Kou X, et al.T Cells Are Required for Orthodontic Tooth Movement[J].J Dent Res, 2015, 94(10):1463-1470 [4]He D, Kou X, Yang R, et al.M1-like Macrophage Polarization Promotes Orthodontic Tooth Movement[J].J Dent Res, 2015, 94(9):1286-1294 [5]Jain S, Darveau RP.Contribution of Porphyromonas gingivalis lipopolysaccharide to periodontitis[J].Periodontol, 2010, 54(1):53-70 [6]梁晓菲, 李秋红.牙周病与正畸治疗关系的研究进展[J].大连医科大学学报, 2014, 36(4):393-399 [7]Okamoto A, Ohnishi T, Bandow K, et al.Reduction of orthodontic tooth movement by experimentally induced periodontal inflammation in mice[J].Eur J Oral Sci, 2009, 117(3):238-247 [8]Gomes SC, Varela CC, da Veiga SL, et al.Periodontal conditions in subjects following orthodontic therapy[J].A preliminary study. Eur J Orthod, 2007, 29(5):477-481 [9]Bollen AM.Effects of malocclusions and orthodontics on periodontal health: evidence from a systematic review[J].J Dent Educ, 2008, 72(8):912-918 [10]Li C, Li B, Dong Z, et al.Lipopolysaccharide differentially affects the osteogenic differentiation of periodontal ligament stem cells and bone marrow mesenchymal stem cells through Toll-like receptor 4 mediated nuclear factor κB pathway[J].Stem Cell Res Ther, 2014, 5(3):67-67 [11]Matsuda S, Movila A, Suzuki M, et al.A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis. J Immunol Methods, 2016, 438:21-25.[J].J Immunol Methods, 2016, 438(1):21-25 [12]孙聪, 刘文佳, 金岩, 等.小鼠正畸牙齿移动模型的制作[J].牙体牙髓牙周病学杂志, 2017, 27(5):280-283 [13]Krishnan V, Davidovitch Z.On a path to unfolding the biological mechanisms of orthodontic tooth movement[J].J Dent Res, 2009, 88(7):597-608 [14]Feller L, Khammissa RA, Schechter I, et al.Biological Events in Periodontal Ligament and Alveolar Bone Associated with Application of Orthodontic Forces. ScientificWorldJournal, 2015, 2015:876509.[J].ScientificWorldJournal, 2015, 1(2015):876509-876509 [15]余岚, 葛楠, 陈莉.不同牙周状态下正畸力诱导和细胞的表达及其比率变化的初步研究[J].北京口腔医学, 2017, 25(2):76-80 [16]Zeng M, Kou X, Yang R, et al.Orthodontic Force Induces Systemic Inflammatory Monocyte Responses[J].J Dent Res, 2015, 94(9):1295-1302 [17]Antoun JS, Mei L, Gibbs K, et al.Effect of orthodontic treatment on the periodontal tissues[J].Periodontol, 2017, 74(1):140-157 [18]陈莉, 李小彤, 范晓川, 等.不同牙周状态正畸力诱导破骨细胞分化因子在牙周组织的表达[J].北京口腔医学, 2015, 23(2):61-64 [19]肖立伟, 陈扬熙, 白丁.正畸力作用下牙周炎大鼠牙周膜骨保护素及配体的-表达[J].华西口腔医学杂志, 2007, 25(5):497-503 [20]He D, Kou X, Luo Q, et al.Enhanced M1M2 macrophage ratio promotes orthodontic root resorption[J].J Dent Res, 2015, 94(1):129-139 [21]Gorbunkova A, Pagni G, Brizhak A, et al.Impact of Orthodontic Treatment on Periodontal Tissues: A Narrative Review of Multidisciplinary Literature. Int J Dent, 2016, 2016:4723589.[J].Int J Dent, 2016, 1(2016):4723589-4723589 [22]朱英, 石飞.牙周—正畸联合治疗牙周炎患者例疗效观察[J].医学美学美容, 2013, 2(2):76-77 [23]Fisher MA, Wenger RM, Hans MG.Pretreatment characteristics associated with orthodontic treatment duration[J].Am J Orthod Dentofacial Orthop, 2010, 137(2):178-186 [24]Sayers MS, Newton JT.Patients' expectations of orthodontic treatment: part 2--findings from a questionnaire survey[J].J Orthod, 2007, 34(1):25-35 [25]Shirazi M, Ahmad Akhoundi MS, Javadi E, et al.The effects of diode laser (660 nm) on the rate of tooth movements: an animal study[J].Lasers Med Sci, 2015, 30(2):713-718 [26]Al-Daghreer S, Doschak M, Sloan AJ, et al.Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption in beagle dogs[J].Ultrasound Med Biol, 2014, 40(6):1187-1196 [27]Yadav S, Dobie T, Assefnia A, et al.Effect of low-frequency mechanical vibration on orthodontic tooth movement[J].Am J Orthod Dentofacial Orthop, 2015, 148(3):440-449 [28]Qamruddin I, Alam MK, Khamis MF, et al.Minimally Invasive Techniques to Accelerate the Orthodontic Tooth Movement: A Systematic Review of Animal Studies. Biomed Res Int, 2015, 2015:608530.[J].Biomed Res Int, 2015, 1(2015):608530-608530