CKS2在口腔鳞状细胞癌中的表达及其生物学意义

口腔生物医学 ›› 2023, Vol. 14 ›› Issue (4): 233-238.

CKS2在口腔鳞状细胞癌中的表达及其生物学意义

冯星雨,张伟,赵雅*

南京医科大学附属淮安第一医院口腔科

收稿日期:2023-04-17 修回日期:2023-06-25 出版日期:2023-12-25 发布日期:2023-12-28
通讯作者: 赵雅 E-mail:zysmile0915@163.com
基金资助:
南京医科大学科技发展基金

Expression of CKS2 and its effect on cell biological behavior in oral squamous cell carcinoma

Received:2023-04-17 Revised:2023-06-25 Online:2023-12-25 Published:2023-12-28
Contact: Ya -Zhao E-mail:zysmile0915@163.com

摘要/Abstract

摘要： 目的：探索口腔鳞状细胞癌（OSCC）中细胞周期蛋白依赖性激酶调节亚基2（CKS2）的表达模式及OSCC细胞系Cal27中CKS2表达对细胞表型的影响。方法：通过生物信息学分析探索CKS2 mRNA在OSCC及正常黏膜组织中的表达水平；免疫组化染色（IHC）检测91例OSCC患者标本和20例口腔正常上皮黏膜中CKS2的表达水平，探究CKS2表达在OSCC患者中的预测价值；siRNA靶向敲减Cal27细胞CKS2并通过Western blot验证其敲低效率，CKS2沉默后通过细胞克隆形成实验检测CKS2对Cal27细胞增殖的影响；通过周期实验和划痕实验分别检测其对细胞周期及迁移的影响。结果：生物信息学分析及IHC结果均显示，CKS2在OSCC组织中高表达（P＜0.05）；CKS2表达水平与患者肿瘤大小、颈淋巴结转移及患者生存率显著相关（P＜0.05）；靶向抑制CKS2表达抑制Cal27细胞的增殖及迁移能力，诱导G₂期阻滞（P＜0.05）。结论：CKS2在OSCC中高表达，其高表达可用来判断患者的生存质量，并正向调控OSCC细胞的增殖、周期及迁移。

Abstract:

Objective: To investigate the expression pattern of cyclin-dependent kinase regulatory subunit 2 (CKS2) and its effects on cell biological behavior in patients with oral squamous cell carcinoma (OSCC). Methods: Bioinformatics analysis was conducted to explore the expression level of CKS2 mRNA in OSCC and normal mucosal tissues. We selected 91 OSCC and 20 healthy oral mucosa samples. Immunohistochemical (IHC) staining was used to detect the expression level of CKS2 in 91 specimens of OSCC patients and 20 normal oral epithelial mucosa, and to explore the predictive value of CKS2 expression in OSCC patients. Small interfering RNA (siRNA) was used to silence CKS2 of Cal27 cells and its knockdown efficiency was determined by western blot. The effects of CKS2 silencing on the proliferation, cycle and migration of Cal27 cells were detected by cell clone formation assay, cycle assay and wound healing assay. Results: Bioinformatics analysis and IHC results showed that the expression of CKS2 in OSCC tissues was significantly higher than that of normal oral mucosa (P＜0.05). The expression of CKS2 was correlated with tumor size, cervical lymph node metastasis and survival rate of patients (P＜0.05). CKS2 silencing inhibited the proliferation and migration of Cal27 cells and induced G₂phase arrest (P＜0.05). Conclusions: Our findings revealed that CKS2 was overexpressed in OSCC, its high expression relating to the poor prognosis of OSCC patients, and involving in the positive regulation of OSCC cell proliferation, migration and cycle.

冯星雨张伟赵雅. CKS2在口腔鳞状细胞癌中的表达及其生物学意义[J]. 口腔生物医学, 2023, 14(4): 233-238.

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