白藜芦醇/介孔二氧化硅/双相磷酸钙复合微球的制备及特性

口腔生物医学 ›› 2025, Vol. 16 ›› Issue (3): 141-149.

白藜芦醇/介孔二氧化硅/双相磷酸钙复合微球的制备及特性

孙素¹,²,颜廷亭³,彭浩然³,晏莺²,张婧婷²,刘彦⁴,周静⁵

1. 昆明市中医医院
2. 云南中医药大学第三附属医院
3. 昆明理工大学
4. 昆明医科大学附属口腔医院
5. 昆明医科大学附属延安医院

收稿日期:2024-11-04 修回日期:2024-12-10 出版日期:2025-06-25 发布日期:2025-06-30
通讯作者: 周静 E-mail:458969533@qq.com
基金资助:
云南省科技厅基础研究计划基金资助项目;云南省科技厅基础研究计划基金资助重点项目;云南省科技创新人才培养项目

Preparation and characteristics of resveratrol/mesoporous silica/bipolar calcium phosphate composite microspheres

1.
2. The Third Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,
3. Kunming University of Science and Technology
4. The Third Affiliated Hospital of Yunnan University of Traditional Chinese Medicine
5. The Stomatological Hospital Affiliated to Kunming Medical University
6. Yan 'an Hospital affiliated to Kunming Medical University

Received:2024-11-04 Revised:2024-12-10 Online:2025-06-25 Published:2025-06-30
Contact: Jing Zhou E-mail:458969533@qq.com

摘要/Abstract

摘要： 目的：制备白藜芦醇（Res）/介孔二氧化硅（MSNs）/双相磷酸钙（BCP）复合微球，检测其理化特性和生物相容性。方法：采用乳液聚合和硬模板法制备MSNs空心微球并负载Res和BCP，通过透射电镜（TEM）和扫描电镜（SEM）观察复合微球支架的微观结构，通过比表面积及孔隙度测试分析微球的比表面积及孔径分布，通过傅里叶红外光谱（FTIR）测试分析支架的内部化学结构，通过X射线衍射测试（XRD）分析支架的物质组成，通过药物释放性能测试Res药物释放量，通过降解性能测试分析支架的降解能力。将复合微球支架浸提液与人牙髓干细胞（hDPSCs）共培养后，通过CCK-8检测细胞活力，通过SEM观察细胞黏附评估复合微球支架对hDPSCs的生物相容性。结果：TEM观察到MSNs的分散性较好，SEM观察到MSNs与BCP形成良好的结合。比表面积及孔隙度测试结果表明微球具有超小尺寸的介孔孔径和较大的比表面积。FTIR分析结果显示复合微球支架中存在羟基磷灰石（HAp）、β-磷酸三钙（β-TCP）、Res和二氧化硅（SiO2）。XRD图谱表明Res/MSNs/BCP支架材料中有HAp、β-TCP和SiO2的特征峰。不同质量Res药物释放测试结果显示，随着时间延长释放浓度逐渐平稳，随着负载的Res质量增加释放量增大（P＜0.05）。降解实验结果显示MSNs的降解速率最慢，而BCP降解速率较快，MSNs/BCP的降解速率介于MSNs和BCP之间，初期降解速度较快，21 d后降解率逐渐平稳（P＜0.05）。CCK-8结果显示，负载不同质量Res的复合支架材料的1.0%浸提液无细胞毒性，随着浸提液浓度增加hDPSCs细胞活力降低（P＜0.05）。细胞黏附结果显示，复合微球支架材料的表面结构适合hDPSCs的黏附和生长。结论：Res/MSNs/BCP复合微球支架具有良好的表征及理化性，无细胞毒性，生物相容性良好。

关键词: 牙周炎, 白藜芦醇, 介孔二氧化硅, 双相磷酸钙, 复合微球

Abstract: Objective:To prepare resveratrol（Res）/mesoporous silica nanoparticles（MSNs）/bipolar calcium phosphate (BCP) composite microspheres and to test their physicochemical properties and biocompatibility. Methods： MSNs hollow microspheres were prepared by emulsion polymerization and hard template method and loaded with Res and BCP, the microstructure of the composite microsphere scaffold was observed by transmission electron microscope (TEM) and scanning electron microscope (SEM), the specific surface area and pore size distribution of the microsphere were analyzed by specific surface area and porosity test, the internal chemical structure of the scaffold was analyzed by fourier transform infrared spectroscopy (FTIR), the material composition of the scaffold was analyzed by Xray diffraction (XRD), the drug release amount of Res was tested by drug release performance test, and the degradation performance of the composite microsphere scaffold was analyzed by degradation performance test. Human dental pulp stem cells (hDPSCs) were co-cultured with the composite microsphere scaffold extract, the cell viability was detected by CCK-8, and the cell adhesion was observed by SEM to evaluate the biocompatibility of composite microspheres scaffolds for hDPSCs. Results： The dispersion of MSNs was good under TEM, and the combination of MSNs and BCP was good under SEM. The test results of specific surface area and porosity showed that the composite microspheres had ultrasmall mesoporous pore size and large specific surface area. The results of FTIR analysis showed the presence of hydroxyapatite (HAp), β-tricalcium phosphate (β-TCP), Res and silica (SiO2) in the composite microsphere scaffold. XRD patterns showed that the characteristic peaks of HAp, β-TCP and SiO2 in Res/MSNs/BCP scaffold materials. The results of drug release test of different quantities of Res showed that the release concentration gradually stabilized with the extension of time, and the release amount increased with the increase of Res weight (P＜0.05). The degradation experiment results showed that the degradation rate of MSNs was the slowest, while the degradation rate of BCP was faster, the degradation rate of MSNs/BCP was between that of MSNs and BCP, and the degradation rate was faster at the initial stage, and gradually stabilized after 21 days (P＜0.05). The results of CCK-8 showed that 1.0% extract of composite scaffold loaded with different Res had no cytotoxicity, and the viability of hDPSCs cells decreased with the increase of extract concentration (P＜0.05). The results of cell adhesion showed that the surface structure of composite scaffold material was suitable for the adhesion and growth of hDPSCs. Conclusions： Res/MSNs/BCP composite microspheres scaffolds have good characterization, physicochemical properties, no cytotoxicity and good biocompatibility. 

Key words: Periodontitis, Resveratrol, Mesoporous silica, Biphasic calcium phosphate, Composite microspheres

孙素颜廷亭彭浩然晏莺张婧婷刘彦周静. 白藜芦醇/介孔二氧化硅/双相磷酸钙复合微球的制备及特性[J]. 口腔生物医学, 2025, 16(3): 141-149.

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