miR-9对人牙周膜干细胞功能的影响

Abstract

Abstract: Objective：To observe the effects of miR-9 on the proliferation, migration and osteogenic differentiation of human periodontal ligament stem cells(PDLSCs) in vitro. Methods： PDLSCs were isolated and cultured in vitro, and were transfected with miR-9 inhibitor. Proliferation of PDLSCs was evaluated by Brdu assay. Migration of PDLSCs was tested by cell wound scratch assay. Osteogenic differentiation of PDLSCs was determined by Alizarin red staining and Western blot. Results： Suppression of the function of miR-9 could reduce proliferation of PDLSCs, lowered the migration speed of PDLSCs, and harmed the osteogenic differentiation of PDLSCs (P＜0.05). Conclusions： miR-9 could regulate the proliferation, cell migration and osteogenic differentiation of PDLSCs.

Key words: miR-9, periodontal ligament stem cells, proliferation, cell migration, osteogenic differentiation

References 11

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Effects of miR-9 on the function of human periodontal ligament stem cells

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