全反式维甲酸联合阿帕替尼抑制口腔鳞癌细胞增殖体外研究

Abstract

Abstract: Objective:To investigate the effects of all-trans retinoic acid(ATRA) and Apatinib on the proliferation of oral squamous cell carcinoma(OSCC). Methods:The effect of ATRA and Apatinib on the proliferation was detected by MTT assay in CAL27 and HN4 cell. The morphology changes of cells were observed by inverted microscope. Relative mRNA expression of KRT1, KRT10 and the stemness genes and IGF1/IGF1R signaling pathway related genes in OSCC were compared by quantitative real time RT-PCR. Results: ATRA and Apatinib inhibited OSCC proliferation, while the cell growth inhibition in ATRA combined with Apatinib group was more significantly than ATRA group or Apatinib group(P<0.05). A series of cell morphological changes was observed in the combined treatment group. ATRA combined with Apatinib significantly upregulated the expression of KRT1 and KRT10, promoting cell senescence(P<0.05). ATRA combined with Apatinib decreased the expression of stemness genes, eliminating the stemness of OSCC, and inhibited the activity of IGF1/IGF1R signaling pathway (P<0.05). Conclusions:The double roles of ATRA combined with Apatinib in OSCC: promoting cell senescence and eliminating the stemness of OSCC stem cell and thus suppressing cell proliferation.

CLC Number:

R739.8

References 28

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In vitro Study on the Inhibition of oral squamous cell carcinoma Induced by All-Trans Retinoic Acid and Apatinib

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