Oral Biomedicine ›› 2022, Vol. 13 ›› Issue (2): 86-91.

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Study of salivary microbiome in autism spectrum disorders children

  

  • Received:2021-12-09 Revised:2022-03-01 Online:2022-06-25 Published:2022-07-07
  • Contact: Sun DeGang E-mail:sundegang168@sina.com

Abstract: Objective:The salivary microbial communities in healthy and caries children with autism spectrum disorders were compared by third high-throughput sequencing platform combined with metagenomics technique. Methods:Saliva samples were collected from 22 healthy children (Caries-free, CF group) and 20 high caries (Caries-high, CH group) children with autism spectrum disorders (ASD), and the metagenomic DNA was extracted and amplified by polymerase chain reaction (PCR). The full-length 16S rRNA gene was sequenced by Pacbio SMRT sequencing technique, and the difference of salivary microbial structure and composition was analyzed and compared between two groups. Results:The difference in microbial structure between the CF and CH group was statistically significant (P<0.05). Moreover, the community structure in CH group was more similar and conserved (P<0.05). The microbial composition analysis results identified three health-enriched taxa, namely, Bergeyella spp, Prevotella nanceiensis, and Staphylococcus aureus for CF group (P<0.05), and four caries-enriched taxa including Streptococcus mutans, Prevotella oris, Dialister invisus and Fusobacterium nucleatum for CH group at species level (P<0.05). Conclusions:The study characterized the caries-specific community structure and biomarkers of the oral microbiota in ASD children, which might help develop novel strategies for the prevention and diagnosis of dental caries in ASD children population.

Key words: autism spectrum disorders, dental caries, saliva microbiome, metagenome, Pacbio SMRT high-throughput sequencing