根尖牙乳头干细胞外泌体通过AHR调节CDC42转录促进血管内皮细胞迁移的机制研究

Abstract

Abstract: Objective:To explore the potential mechanism by which exosomes derived from stem cells from apical papilla (SCAP-Exo) regulate the migration of vascular endothelial cells (VECs) by targeting cell division cycle 42 (CDC42) transcription. Methods:?SCAP-Exo extracted through ultracentrifugation were characterized by transmission electron microscopy and nanoparticle tracking analysis. VECs transfected with siCDC42 were treated with SCAP-Exo, and real-time quantitative PCR and western blot assays were conducted to detect the mRNA and protein expression levels of CDC42 after SCAP-Exo treatment. Scratch wound healing and transwell cell migration assays were performed to assess the migration of VECs. The effects of SCAP-Exo on aryl hydrocarbon receptor (AHR) nuclear translocation and CDC42 expression in VECs were detected by western blotting and immunofluorescence staining assays. Results:?SCAP-Exo could upregulate the mRNA and protein expression levels of CDC42 and enhance the migration of VECs compared to those in the control VECs group. The migration rate of VECs were reduced when VECssiCDC42 were treated with SCAP-Exo compared to the SCAP-Exo-treated VECs. SCAP-Exo induced AHR translocation to the nucleus in VECs. After inhibiting AHR nuclear translocation in VECs, the mRNA and protein expression levels of CDC42 decreased. Conclusions:SCAP-Exo promoted CDC42 transcription by activating AHR translocation, thereby upregulating CDC42 expression, which promoted the migration of VECs. Conclusions:?SCAP-Exo promoted CDC42 transcription by activating AHR translocation, thereby upregulating CDC42 expression, which promoted the migration of VECs.

Key words: Stem cells from apical papilla, Exosome, Vascular endothelial cell, Cell migration, Cell division cycle 42, Aryl hydrocarbon receptor

References 22

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Exosomes derived from stem cells from apical papilla promotes the migration of vascular endothelial cells by regulating CDC42 transcription via AHR

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