主穹隆蛋白通过抑制破骨细胞分化在牙周炎中起骨保护作用

›› 2020, Vol. 11 ›› Issue (1): 24-27.

主穹隆蛋白通过抑制破骨细胞分化在牙周炎中起骨保护作用

赵娜¹,袁礼婵²,郭舒瑜¹,马俊青³

1. 南京医科大学口腔疾病研究江苏省重点实验室，南京医科大学
2. 南京医科大学附属口腔医院
3. 南京医科大学口腔疾病研究江苏省重点实验室

收稿日期:2019-11-14 修回日期:2020-01-17 出版日期:2020-03-25 发布日期:2020-04-07
通讯作者: 马俊青 E-mail:jma@njmu.edu.cn
基金资助:
国家自然科学基金;江苏省高校自然科学研究重大项目;江苏高校优势学科建设工程资助项目

MVP plays a protective role in periodontitis by inhibiting osteoclast differentiation and reducing bone resorption

Received:2019-11-14 Revised:2020-01-17 Online:2020-03-25 Published:2020-04-07

摘要/Abstract

摘要： 目的：通过建立小鼠实验性牙周炎模型及体外骨髓间充质干细胞（BMMSCs）破骨细胞向诱导，探讨主穹隆蛋白（MVP）在牙周炎骨吸收中的作用。方法：MVP基因敲除（MVP-/-）和野生型（WT）C57BL/6小鼠分别局部注射脂多糖（LPS）以建立实验性牙周炎模型，通过micro CT扫描、耐酒石酸酸性磷酸酶（TRAP）染色等方法检测骨吸收程度。同时，体外分离培养MVP-/-与WT C57BL/6小鼠的BMMSCs，并诱导其向破骨细胞分化，通过TRAP染色、麦胚凝集素（WGA）染色等方法观察MVP对BMMSCs破骨向分化及骨吸收活性的影响。结果：在LPS诱导的小鼠实验性牙周炎中，MVP-/-组小鼠牙周炎骨吸收更为明显，且在注射区域内可见更多破骨细胞；体外实验证明，MVP-/-组小鼠的BMMSCs分化形成更多的破骨细胞，且骨吸收更明显。结论：MVP可以抑制破骨细胞分化，在牙周炎中起骨保护作用。

关键词: 牙周炎, 主穹隆蛋白, 破骨细胞, 分化, 骨吸收

Abstract: Objective: To investigate the role of major vault protein (MVP) in periodontitis by establishing an experimentalmodel of periodontitison mice and osteoclast induction of bone marrow mesenchymal stem cells (BMMSCs) in vitro. Methods:A model of experimental periodontitis was established by local injection of lipopolysaccharide (LPS) into wild-type mice (WT) and MVP knockout (MVP-/-) mice.The severity of periodontitis was detected by micro-CT and TRAP staining. In vitro, the role of MVP in osteoclast differentiation was verified by TRAP staining and Wheat Germ Agglutinin (WGA) staining after cultured with RANKL and M-CSF. Results:In experimental periodontitis induced by LPS, MVP-/- group developed more bone resorption and more osteoclasts could be seen in the injection area. In vitro experiments showed that knockout MVP promoted osteoclast differentiation and bone resorption.Conclusions:MVP plays a protective role in periodontitis by inhibiting osteoclast differentiation.

Key words: MVP, osteoblast, differentiation, bone resorption

赵娜袁礼婵郭舒瑜马俊青. 主穹隆蛋白通过抑制破骨细胞分化在牙周炎中起骨保护作用[J]. 口腔生物医学, 2020, 11(1): 24-27.

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