Abstract: Tooth loss is one of the most common diseases, and the existing clinical restorative methods fail to achieve functional tooth regeneration. With the advancement of regenerative medicine, stem cell-based tissue engineering techniques have emerged as promising therapeutic strategies.The tooth root is the basis for the stable function of the tooth. Therefore, a key challenge in tooth regeneration lies in the successful regeneration of functional tooth roots. By collecting tissues in vitro to obtain stem cells with high proliferation and differentiation potential, and combining them with biocompatible scaffold materials, it is possible to construct tissue-engineered biological roots (bio-root). This review aims to provide an overview of recent advancements in the research on biological tooth roots.

Key words: Bio-root, Regenerative medicine, Tissue engineering, Stem cells