Objective：To isolate and culture the oral cancer associated fibroblasts (CAFs) and explore the possible role of CAFs in the invasion and metastasis of oral cancer cells in the tumor microenvironment. Methods：The tissue block culture method and enzymatic digestion method were used in the isolation, culture and purify of oral CAFs and normal mucous fibroblasts (NFs). And then the immunofluorescence assay and Western blot were used to identify the NFs and CAFs. Results：The oral CAFs were successfully isolated and cultured. They were long fusi form in shape, and the cells were in dense aggregations. The CAFs grew at certain point into multilayer structure and arranged in disorder. The cytokeratin 18 (CK18) was negative in NFs and CAFs, while the Vimentin was positive. The α-smooth muscle actin (α-SMA), fibroblast specific protein-1 (FSP-1), fibroblast activation protein (FAP), and platelet derived growth factor receptor α(PDGFR-α) showed positive in CAFs compared to the NFs. The Desmin were expressed in NFs and CAFs. In the protein levels, the α-SMA, PDGFR-α were significantly increased in CAFs as compared to that in NFs. In addition, the FSP-1 was decreased in CAFs. However, there was no significant difference in the of Desmin and FAP in NFs and CAFs. Conclusions：In morphology, the oral CAFs and NFs were significantly different in growth and protein .

Objective：By overexpressing the oligodendrocyte lineage-myelin basic protein (Golli- MBP) gene to induce oral mucosa to produce oral lichen planus (OLP) lesions and explore the role of genes of Golli-MBP in the pathogenesis of OLP. Methods：By using lentiviral vectors to mediate Golli-MBP over, which was injected under Syrian golden hamsters’ oral mucosa, we detected mucosal changes by visual observation , histological changes by HE staining, cell apoptosis by TUNEL assay, and cell proliferation by immunohistochemical method. Results：Golli-MBP failed to induce OLP lesions of experimental animals. There was no significant difference (P>0.05) of apoptosis rate in experimental groups and the control groups in the epithelia lamina on the 7th day and the apoptosis rate on 14th day in the experimental groups was lower than that of the control groups, the difference was statistically significant (P<0.05). Cortical cell proliferation rate on the 7th day and 14th day in the experimental groups was lower than that of the control groups and the difference was statistically significant (P<0.05). Conclusions：Golli-MBP over can suppress the proliferation and apoptosis of Syrian golden hamster cheek pouch mucosal epithelial cells. However, we failed to induce it to produce OLP lesions.

Objective：To investigate whether or not the TNF-α can affect mouse bone marrow stromal stem cells (mBMSCs)osteoporosis through miR-21.Methods：Real-time RT-PCR was used to detect the levels of miR-21 in mBMSCs osteogenesis on condition of TNF-α. Osteogenic gene and protein was determined by real-time RT-PCR and Western blot analysis, respectively. After upregulation the of miR-21 in the presence of TNF-α, the osteogenic potential of mBMSCs were investigated. Results：TNF-α inhibits mBMSCs osteoporosis and miR-21 can restore the potential of mBMSCs treated with TNF-α. Conclusions：TNF-α inhibits mBMSCs osteoporosis through miR-21.

Objective：To construct the over RAS1 gene of Candida albicans. Methods：We put the open reading frame of RAS1 gene under the control of MET3 promoter in plasmid pCaEXP to construct recombinant plasmid pCaEXP-RAS1 which can overexpress RAS1 gene. The plasmid pCaEXP-RAS1 was transformed into Escherichia coli DH5α for amplification and extracted by Plasmid Maxi Kit. Then the recombinant plasmid was linearized by cutting of restriction enzyme, and the linear recombinant plasmid was introduced into Candida albicans CAI4 by lithium acetate to select positive colonies on the plates of SD-ura-met-cys selective culture medium. Realtime RT-PCR was used to detect the level of mRNA of RAS1gene. Results：RAS1-over plasmid was exactly established by restriction enzyme digestion. RAS1-over strain was constructed as confirmed by quantitative real-time PCR. RAS1-over strain was selected by quantitative real-time PCR. Conclusions：The RAS1-over strain can be successfully constructed by recombinant pCaEXP-RAS1 plasmid.

Objective：To investigate the and clinical significance of tumor-associated macrophages(TAMs) and matrix metalloproteinase-2(MMP-2) in ameloblastomas（ABs）. Methods： The of TAMs(CD68) and MMP-2 was examined in 43 ABs and 10 normal oral mucosa by universal two-step immunohistochemical technique. Results：Expression of TAMs and MMP-2 were significantly higher in ABs compared with normal oral mucosa(P<0.01). TAMs was mainly expressed within the stroma and MMP-2 was mainly expressed within the islands of ABs. There was a positive correlation between MMP-2 score and TAMs count in ABs(r=0.331, P<0.05). Conclusions：The common of TAMs and MMP-2 may play an important role in the development of ABs.

Objective：To study the of MageD1 in oral squamous cell carcinoma (OSCC) and HN6 cell lines and to explore its role in promoting cell apoptosis through the transfection of MageD1 in the oral squamous cell carcinoma cell line HN6. Methods：The of MageD1 in OSCC and HN6 cell lines are tested by Western blot and RT-PCR, and Cleaved-Casepase3 and Bax were tested by Western blot through the transfection of MageD1 in the cell line HN6, and study the growth of cell line HN6 transfected with MageD1 in nude mice. Results：The of MageD1 in OSCC and HN6 cell lines are both lower than normal tissues. MageD1, Cleaved-Casepase3 and Bax were upregulated in the Lv-Maged1-HN6 cells than the HN6 cells. The implantation experiment showed that tumors formed by the Lv-Maged1 cells are smaller than those by HN6 cells in nude mice. Conclusions：MageD1 is down-regulated in the OSCC and the over- of MageD1 promotes cell apoptosis.

Objective: To investigate the effect of thermocycling on the crystal structure of zirconia and measure the fracture resistance of zirconia cantilever fixed bridge with 0.7mm and 0.8mm in the posterior dentition. Methods: Two 10×15×1mm zirconia specimens (one served as control and another subjected to thermocycling, 100000 times, 5-55℃ ) were prepared. X-ray diffraction (XRD) was used to estimate the relative amount of monoclinic phase in each specimen. 12 zirconia cantilever fixed bridge frameworks were prepared by using CAD/CAM systems and divided into two groups (0.7mm and 0.8 mm, 6 in each group). All frameworks were cemented on the model using glass ionomer luting cement and then subjected to compressive test by using a Universal Testing Machine. The loads at fracture were recorded and one-way ANOVA was used to analyze the data. Results: No specimen exhibited the monoclinic phase before and after thermocycling．There was no significant difference in the fracture resistance between group 0.7mm (1262.08±100.57N) and group 0.8mm (1345.87±191.17N) (P>0.05). Conclusions: Thermocycling has no effect on the crystal structure of zirconia. The loads at fracture in both groups were more than the maximum human bite force (888N).

Periodontitis is an inflammatory disease in periodontal tissues caused by dental plaque biofilms. Plaque control plays an important part in periodontal treatment. Some researchers have found that laser associated therapy have the antibacterial effects. Nd:YAG laser is one of this kind of laser that is easily absorbed by pigment tissue and exerts one antibacterial effects. This article reviews the antibacterial effects of Nd:YAG against the periodontal pathogenic bacteria.

Internal resorption is a rare and insidious pathological process. The etiology and the associated key pathogenesis have not been completely understood. Up to date, most studies about internal resorption were case reports, the essence of it is still uncertain. In this paper, we reviewed internal resorption from etiology, molecular biological mechanism, diagnosis and clinical management.

Abstract: Accelerating tooth movement, within the physiological range, can reduce the period of orthodontic treatment, thereby reducing the risks of caries, root resorption, periodontal diseases, etc. Currently, there are various modalities to accelerate tooth movement which can be categorized as follows: surgery, physiotherapy, drugs and gene therapy. The rate of tooth movement depends on the remodeling rate of tissues surrounding the roots. Throughout the clinical and experimental methods now in use, they all act on one of the links in periodontal bone remodeling. This review summarizes the molecular mechanisms of these methods.

Dentists have been paying more and more attention to the study on autotransplantation of cryopreserved teeth.But it’s still a serious problem for us to maintain the pupal vitality in the progress of cryopreservation.Because of the important function of pulp in teeth,vital pulp became a great factor for the success and prognosis of autotransplantation.Influence of apical foramen on the pupal vitality during cryopreservation is reviewed in this paper.Different methods of apicoetomy used in cryopreserving teeth are introduced according to researches.Generally speaking,the effect of apical foramen should be taken into consideration when cryopreservation is needed.In addition,apicoetomy should be a standard operation when donor teeth is almost mature.

Autogenous tooth transplantation is an effective way to repair the missing tooth.It can avoid the critical defects of dental implants.But the time for autogenous tooth transplantation is hard to control at will and it always has the difficulty in its wide application in clinic. The development of biological freezing technology in recent years has been expected to solve these problems. This paper mainly introduced the biological cryopreservation technology of teeth and the present research on cryopreservation of tooth transplantation. The paper also conducted a preliminary introduction on the mechanism of the periodontal and pulpal regeneration after autogenous teeth transplantation,and the influence of cryopreservation on the periodontal and pulpal tissues.