Objective：To develop an animal model of infantile hemangioma by applying conditional transgenic mice. Methods：pTie2-PyMT, a plasmid of Tie2 promoter-driven polyomavirus middle T (PyMT) gene, was constructed. Endothelial specific PyMT gene was transported to male pronucleus of donor C57BL/6Jmice by DNA micro-injection, and the fertilized eggs were transplanted to receptor mice. The founder mice were developed from the surviving fertilized eggs. The gene integration was checked by PCR, and phenotypes of transgenic mice were examined. The neoplasms of transgenic mice were detected by histological method. GraphPad Prism 5.0 software package was used for statistical analysis. Results：The PyMT, Tie2 promoter and Tie2 enhancer were cloned and integrated exactly in pTie2-PyMT plasmid according to sequencing analysis. All the newborn positive transgenic mice had hemangioma phenotypes. The hemangioma-like neoplasms were identified by HE in the ear, tongue, skin surface, mucosa and liver tissue in the transgenic mice, and immunohistology staining showed positive of PyMT and CD31 in endothelial cells. Conclusions：PyMT gene driven by Tie2 promoter can induce hemangioma in mice. Thus, the transgenic mice can be applied as a model system for studying the mechanism of infantile hemangioma. Conditional transgenic technology is an ideal method to develop hemangioma animal model.

Objective：To investigate the effects of parathyroid hormone (PTH) on proliferation and apoptosis ability of bone marrow mesenchymal stem cells (BMMSCs) from ovariectomized（OVX）rats. Methods：An ovariectomized animal model wasadopted. The rats were treated with either subcutaneous PTH or normal saline. The proliferation and apoptosis of BMMSCs were detected by means of flow cytometry. Results:The S-phase fraction of BMMSC in ovariectomized with PTH treatment group （8.87±0.11）% was significantly increased when compared with ovariectomized group（5.23±0.20）%. And the apoptosis of BMMSC in ovariectomized with PTH treatment group（4.17±0.15）% was significantly reduced when compared with ovariectomized group（8.13±0.61）%. Conclusions：Intermittent application of PTH can strengthen the proliferation ability of BMMSCs from OVXrats but suppress the apoptosis.

Objective： To explore the and significance of heat shock factor 1 (HSF1) in 4-nitroquinoline 1-oxide (4NQO) induced rat tongue carcinogenesis. Methods：A total of 80 Wistar rats were randomly divided into two groups. Rats in experiment group were administered orally with 0.001%-0.004% 4NQO for 8-28 weeks, while rats in the control group were administered with tap water. Then the rats were killed at 8, 16, 20, 24, 28 weeks and their tongues were removed for general observation, histological assessment and HSF1 immunohistochemical staining. Results：Gross changes were observed, including granulations, white patches, verruca, cauliflower-like shape leukoplakia and ulcer-like changes on the posterior part of the tongue dorsum of experimental group with increasing concentrations and prolonged action of 4NQO. Their corresponding histopathological results ranged from hyperplasia, mild dysplasia (MiDP), moderate dysplasia (MoDP) and severe dysplasia(SDP), in situ carcinoma (ISC) to early invasive carcinoma (EIC). The incidence of tongue cancer in rats treated with 4NQO for 8, 16, 20, 24, 28 weeks was 0, 20.0%, 50.0%, 66.70% and 100%. Rat tongue mucosa epithelium of the control rats was almost negative or low for HSF1 staining. But with the aggravation of the tongue mucosal dysplasia, the positive of HSF1 of the experimental rats increased obviously. HSF1 was distributed throughout the entire cancer nest in carcinoma in situ. The of HSF1 was low in cancer epithelium of well-differentiated squamous cell carcinoma, while high in carcinoma stromal fibroblasts. Conclusions：4NQO drinking water can induce rat tongue carcinogenesis, wich helps to successfully establish the rat tongue carcinogenesis model and HSF1 may play a key role in the development of the cancer.

Objective：To investigate the effect of simvastatin administration on osseointegration surrounding the implant by establishment of maxillary implant rat model. Methods：Three-month-old female SD rats were randomly divided into control group and oral administration group. The control group was administrated with saline. The other group was administrated with simvastatin. After Titanium implants（0.8 mm×2.0 mm）were put in anterior to the first molar of maxillary, different administration programs were applied and the rats weights and serum concentrations of ALP and BGP were detected at 1, 2 and 4 weeks, respectively. Two weeks after administration, HE staining was used to observe the formation of new bone. After 4 weeks administration, micro-CT was used to observe the bone structure by measuring bone density around the implant. Results： The weight of rats was decreased in simvastatin administration group compared with that of the control group, and the difference was statistically significant (P< 0.05). The data of serum ALP and BGP showed the similar trend with weight (P< 0.05). At 2nd week, HE staining indicated that much more trabecular bone was observed in simvastatin administration rats in contrast with control. At 4th week, the data of all the trabecular bone parameters were increased in simvastatin administration rats, except for Tb.Sp and the difference was statistically significant (P< 0.05). Meanwhile, three-dimensional reconstruction also confirmed the similar results. Conclusions：Simvastatin significantly promotes the formation of new bone around the implant via orally administration.

Objective: To study the level of miR-595 in serum of patients with mandibular prognathism during growth period and its relationship with mandibular growth and ossification. Methods: The research chose 83 skeletal class Ⅲ malocclusion (23 mixed dentition, 60 early permanent dentition) and 92 normal controls (24 mixed dentition, 68 early permanent dentition), using RT-PCR to detect miR-595 levels in two groups and TargetScan software to predict its possible target genes. Results: Expression of miR-595 in mandibular prognathism group was down regulated. There was negative correlation between miR-595 and mandibular growth with statistical differences (P＜0.01). Conclusions: miR-595 might have inhibitory effect on mandibular growth and ossification through target gene.

Objective: To tackle the dimensional shrinkage of electrospun membrane of (poly(lactic-co-glycolic acid), PLGA) by adding a certain amount of (poly(ε-caprolactone), PCL)in order to make it qualify for clinical usewith the biocompatibility of PLGA not significantly affected. Methods: We blended PLGA and PCL in different weight ratio (10:0, 7:3, 6:4, 5:5 and 0:10), and fibrous membranes were collected by electrospun. The diameters and shrinkage of the fibers were compared. The biocompatibilities of different membranes were compared by culturing osteoblasts under the same condition on them. The topography of the membranes and the morphology of the cells adherent on different membranes were observed with scanning electron microscope. Results: The diameter of electrospun fibers rose as the ratio of PCL increased, but the topography of the membranes and the morphology of the cells adherent on them presented no significant diversity among different PLGA/PCL ratio. The biocompatibility of PLGA declined with the adding of PCL, and different blending groups (7:3, 6:4, 5:5) showed no statistical differences, but they were still better than PCL. PLGA electrospun membranes showed significant shrinkage in phosphate buffer solution, and the dimensional shrinkage decreased with the addition of PCL, but the decrease presented no significant differences among different PLGA/PCL ratio. Conclusions: Blending PLGA/PCL at a certain ratio can prominently ameliorate the shrinkage of PLGA, furthermore, the blending has little influence on the excellent biocompatibility of PLGA.

Objective: To observe the remodeling of alveolar bone in rats with the vertical absorption after loading orthodontic force, and to provide evidence for clinical orthodontic treatment. Methods: 75 SD rats (10-week-old ,male)were randomly divided into three groups(25 in each group):loading force control group (A), control group of periodontitis with vertical bone absorption (B), loading force group to periodontitis with vertical bone absorption (C).With loading force on for 8 hours, 1d,7d, 14d and 21d,the alveolar bone of the first molar of left maxillary were taken to do the histological and immunological detection. Results: On the7th day, the derange of the periodontal ligament fibers, non-cellular structure, a small amount of inflammatory cells,and active multinucleated osteoclasts were observed. There was no significant difference between the control groups and experimental group in histological detect. But the IGF-1 peak was detected on the 7th day in experimental group(C),much higher than that in the control groups(P<0.05).On the 14th day, the RUNX2 peak was detected in the experimental group(C), and much higher than that in the control groups(P<0.05).Conclusions: After controlling periodontal disease and eliminating occlusal trauma, loading orthodontic force which could enhancethe of RUNX2 and IGF-1in alveolar bone defect region, and also the capacity of remodeling of bone matrix and collagen, contribute to tooth alveolar bone remodeling.

Objective: To prepare polymer-polyvinyl alcohol(PVA)and polyacrylamide(PAM) hydrogel used for oral soft tissue expansion. Methods: PVA and PAM were dissolvedat a high temperature and frozen in a low temperature and then melted at room temperature. After being dried in the vacuum oven ,and cut into cubes which were 18 mm*5 mm*4 mm in volumewith the concentration of15wt%,20wt% and 25wt% respectively. The dry gel of PVA and PAM were characterized. Results: The dry gel of PVA was hard, homogeneous and smooth on the surface. But the dry gel of PAM could be found small holes on the surface and was fragile, uneven and rough. Conclusion: A small amount of PVA and PAM could be dissolved in deionized water through stirring at room temperature, but PVA and PAM could be completely dissolved at a higher temperature. PVA could be solidified through repeated freezing and unfreezing for several times, and become dry gel by vacuum drying , which woud be shrunken two or three times in the volume.

Objective: The purpose of this study was to explore the influences of different cooling methods and cryoprotectants on the viability of periodontal ligament cells in vitro. Methods: A total of 25 fresh teeth (the first premolar or second premolar) were collected and randomly divided into five groups. Four experimental groups were cooled to -196 ℃ by programmed freezer or rapid freezer in cryoprotectants with or without trehalose for 1 week. In the control group, the fresh teeth were not given any treatment. The PDL attached to the middle third of roots was shaved from the root surface using scalpel knife. Filtered fluid was collected after digestion using trypsin for 5 mins and repeated for 5 times. After centrifugation, the supernatant was discarded. After resuspending the cells with phosphate buffered saline (PBS), the living PDLCs were stained by Trypan blue and counted under high magnification. The cell survival rates of different groups were calculated and statistically analysed. Results: There were no statistically significant difference in survival rates of PDLCs between all groups. But in the group cryopreserved by programmed freezer with trehalose, the survival rates was the highest compared to that of the others. Conclusions: The cryopreservation do not cause obvious damage to the periodontal ligament cells. In our opinion, this technique could be applied in teeth preservation. On the other hand, using the programmed freezer with trehalose in cryoprotectants have minimal effect on the viability of periodontal ligament cells.

The temporomandibular joint is one of the most complex and delicate joints in the human body, and the temporomandibular joint disorders is a common disease in oral and maxillofacial region, which is reported to be about 20% to 40%.Studies have confirmed that large numbers of factors will result in the development of the temporomandibular joint disorders, such as heredity, mental disturbances, aging, the decrease of bone mineral density ,damage, malnutrition and so on. This paper reviews the research on the effect of 1,25-dihydroxyvitamin D 3 on the development and progress of the temporomandibular joint disorders.

Abstract:Mandibular Condylar Cartilage cells(MCCSs) is the only cell components of Condylar Cartilage, and the growth and metabolish of MCCSs is affected by various cytokine, mechanical stimulation and hormone, among which the influence of estrogen hormone has received much concern of the scholars, it might be one of the factors to cause Temporomandibular Joint Disorder. The researches that the estrogen hormone influences the metabolism of Ariticular Chondrocytes are more intensive. But Condylar Cartilage is fibrocartilage, which differ from Ariticular Chondrocytes. The researches about it are relatively lay behind. This paper will give a summary of the interrelated mechanism about estrogen hormone reconstructe the growth, metabolism and applicability of MCCSs, which will contribute to learning more how the change of estrogen level affect the physiological and pathological change of temporomandibular joint.In the hope of making progress in treating Temporomandibular Joint Disorder.